微创法构建猪急性心肌梗死模型

Establishment of a porcine acute myocardial infarction model using minimally invasive procedures

背景:持续稳定的心脏缺血模型是研究心脏血管新生疗法的重要基础,常规"球囊临时堵闭"法存在短期血管再通的缺点,无法评定血管新生治疗的确切疗效。目的:改良常规"球囊临时堵闭"构建心肌梗死模式,探讨一种方便、快捷、安全、可重复的心肌梗死模型制备新方法。方法:将30只健康成年家猪随机分为2组。分别采用"球囊堵闭+栓子植入"法和"栓子直接植入"法构建心肌梗死模型。比较两种方法的造模成功率和导管介入操作时间。结果与结论:实验成功制备了心肌梗死模型27只,"球囊堵闭+栓子植入"法的成功率略低于"栓子直接植入"法(85%vs.94%),两种方法比较造模成功率及梗死部位分布构成差异无显著意义(P>0.05)。但"栓子直接植入"法的导管介入操作时间明显短于"球囊堵闭+栓子植入"法(P<0.01)。说明"栓子直接植入"法建立的猪急性心肌梗死模型具有安全可行、方便快捷、成功率高等优点,并且能较好地模拟临床心肌梗死的发生发展过程,是一种良好的制备心肌梗死模型的方法。

BACKGROUND：A sustained and stable model of cardiac ischemia is an important basis for angiogenesis research.Conventional ＂balloon transient dilation＂ has disadvantage of vascular recanalization after drawing balloon,it is hard to evaluate the therapeutic effect of angiogenesis.OBJECTIVE：To explore a optimize method that could establish the porcine model of acute myocardial infraction safer,quicker,and more convenient than routine methods.METHODS：Thirty healthy,adult pigs were randomly divided into 2 groups.Balloon transient dilation ＋ plug implantation and directly plug implantation were used to construct myocardial infraction models.Intervention operation times and success rates were observed and compared between two groups.RESULTS AND CONCLUSION：A total of 27 pigs underwent induction of myocardial infraction successfully.Success rate of balloon transient dilation ＋ plug implantation group was 84.6%,and that of the directly plug implantation group was 94%,there was not statistic significant compared with two groups（P 0.05）.However,the mean operation time of the directly plug implantation group was shorter than that of the balloon transient dilation＋ plug implantation group（P 0.01）.The myocardial infraction models prepared by directly plug implantation method are characterized by feasible,safe,and relatively effective,which can simulate procedure of clinical myocardial infraction.