组织工程化软骨细胞的定向诱导分化

Directional differentiation of tissue engineered chondrocytes

背景:关节软骨损伤后几乎不能完全修复,在生理负荷下容易发生退行性改变,最终发展成骨性关节炎。因此,利用组织工程化软骨修复重建受损关节软骨为骨关节软骨疾病的治疗开辟了新的途径。目的:全面了解生物特性与正常关节软骨相似的组织工程软骨的构建,明确目前软骨细胞定向诱导分化的研究进展。方法:电子检索中国生物医学文献数据库和计算机Medline数据库1994/2009收录的组织工程化软骨细胞相关综述和论文报告,并分析其诱导种子细胞及调控途径的研究进展。结果与结论:共纳入软骨细胞定向诱导分化相关文献27篇。软骨细胞基质中的生长因子,通过不同的细胞信号通路在软骨生长分化中发挥重要作用,同时周围环境因素也影响诱导分化的结果,但目前对于各个信息通路之间的联系及相互关系尚不完全明确。构建组织化软骨的支架材料也有待进一步发展。

BACKGROUND：Articular cartilage cannot repair completely after injury,which is liable to degenerative change under physiological stress,eventually develops into osteoarthritis.Therefore,repairing damaged articular cartilage using tissue engineered chondrocytes provide a new approach for articular cartilage disease treatment.OBJECTIVE：To understand the biological characteristics and construction of tissue engineered chondrocytes,and to define the progress of directed differentiation of tissue engineered chondrocytes.METHODS：Chinese Biomedical Literature Database and Medline database were retrieved for papers concerning tissue engineered chondrocytes published between 1994 and 2009.The progress of inductive seed cells and regulation pathway were analyzed.RESULTS AND CONCLUSION：Totally 27 literatures were selected.Growth factor in extracellular matrix plays an important role in the growth and differentiation of cartilage via controlling cell signaling pathways.Simultaneously,surrounding environment of chondrocytes also affects cartilage differentiation.However,the relationships among signaling pathways are poorly understood.The scaffold materials for tissue engineered chondrocytes construction need to be improved.