蒙古羊Hoxd11基因CpG位点分布特征分析

Distribution of CpG in Hoxd11 gene for Mongolia Sheep

蒙古羊的Hoxd11基因全长1 772 bp。其中exon-1为778 bp,exon-2为236 bp,内含子为758 bp(Gen-Bank Accession No.GU059862)。Hoxd11 exon-1序列共有120个CpG,其中位于密码第1～2位的CpG共8个,全都编码精氨酸。位于密码第2～3位的CpG共47个,占所有CpG的39.17%(47/120),分别编码5个丝氨酸,18个脯氨酸,2个苏氨酸,22个丙氨酸。Hoxd11 exon-1的CpG数量明显高于第2外显子(12个)。Hoxd11exon-1序列中的高密度CpG,提示这个序列有可能是甲基化的高发区。这对于进一步分析这个基因对调控腰椎发育的作用,具有重要意义。

There were 1 772 bp among the Hoxd11 gene sequence,including 778 bp for the exon-1,236 bp for the exon-2 and 758bp for the intron in Mongolia sheep genome（GenBank Accession No.GU059862）.There were 120 CpGs in the exon-1 and 12 CpGs in the exon-2.This high density of CpG in the exon-1 for Hoxd11 might be meaning the major part of the cytosine methylation occurred this region,and regulating the development of lumbar vertebrae.Because the number of lumbar vertebrae（6 or 7）was controlled by the Hoxd11 for mammals,the methylation in the CpGs of this gene exon-1 could be a molecular marker for early selection in the breed breeding to increase the multi-vertebrae individuals.