酸枣仁汤对老年失眠证候模型大鼠脑皮质超微结构及星形胶质细胞表达的影响

Effects of wild jujube seed decoction on uitrastructure and astrocytes expression in the brain cortex of old model rats with sleep deprivation syndromes

目的 观察睡眠剥夺后酸枣仁汤对老年血亏阴虚失眠证候模型大鼠脑皮质超微结构及星形胶质细胞表达的影响.方法 用D-半乳糖致衰老、环磷酰胺及氢化可的松致阴血亏虚,及自制改良多平台法剥夺睡眠,制作老年血亏阴虚失眠证候大鼠模型.电镜观察实验大鼠大脑皮质超微结构的变化,并用免疫组化方法检测星形胶质细胞标志物-胶原纤维酸性蛋白（GFAP）在大脑皮质部位的表达.结果 与环境对照组比较,证候模型组大脑皮质超微结构改变明显,且GFAP表达阳性细胞数明显增多[（20.4±4.4）个,（9.8±2.5）个,P＜0.01],光密度值明显增大[（0.20±0.01）,（0.11±0.02）,t=7.63,3.18,P＜0.01];而酸枣仁汤高、低剂量组较证候模型组,大脑皮质超微结构有明显改善,且GFAP阳性细胞数减少[（14.4±3.9）个,（15.5±6.4）个],光密度值降低[（0.14±0.02）,（0.14±0.02）]（t=6.32,5.24,2.31,2.45,P＜0.05）,趋向于环境对照组.结论 酸枣仁汤对老年血亏阴虚型失眠证候模型大鼠大脑皮质超微病理结构有明显改善作用,通过调节星形胶质细胞表达是其可能作用机理之一.

Objective To observe the effects of wild jujube seed decoction（WJSD） on ultrastructure and astrocytes expression in the brain cortex of old model rats with blood-deficiency and Yin and sleep deprivation（SD）after SD. Methods Ultrastructural changes in cortical parts of the experiment rat were observed by transmission electron microscopy , immunohistochemical staining was used to detect star-shaped glial cells marker-glial fibrillary acidic protein（GFAP） expression after SD induced by made-self multiple platform method （MMPM）, senescence induced by D-galactose, and Yin and blood-deficiency induced by cyclophosphamide and hydrocortisonum. Results Compared to environmental control group（（9.8 ± 2.5）, （0.11 ± 0.02） ） , syndrome model group （（ 20.4 ±4.4）, （0.20 ±0.011） ） rats had obvious ultrastructural changes and stronger expression of GFAP in cortical parts （t = 7.63,3.18, P 〈 0. 01）, while those of W J SD of high and low dose group （（14.4 ± 3.9）, （15.5 ± 6.4）,（0.14 ±0.02）（0.14±0.02）） rats showed weaker（t=6.32,5.24,2.31,2.45, P〈0.05）. Conclusion WJSD of large and small lose group could improve the rats neurons pathological changes,WJSD downward adjusting the expression of GFAP may be one of the mechanisms of treatment on insomnia with blood-deficiency and Yin in the old.