中国湖北汉族重症肌无力与HLA-Ⅱ类等位基因的关联

Association of HLA class Ⅱ (DRB1/DQB1/DPB1) alleles and myasthenia gravies in the Han nationality of Hubei province

目的和方法：探讨中国湖北汉族重症肌无力（ＭＧ）患者与ＨＬＡ－Ⅱ类等位基因的关联。利用ＰＣＲ／ＳＳＰ和ＰＣＲ－ＲＦＬＰ技术对湖北地区９１例ＭＧ患者进行ＨＬＡ－Ⅱ类（ＨＬＡ－ＤＲＢ１、ＤＱＢ１和ＤＰＢ１）基因型别分析，并与１６８例正常个体比较。结果：患者组（１）：ＨＬＡ－ＤＲＢ１０９０１、ＤＱＢ１０３０３和ＤＰＢ１０５０１等位基因频率明显高于对照组，ＲＲ值分别为４１２、３０４和３０１，Ｐ＜０．０１；（２）ＨＬＡ－ＤＰＢ１０２０２等位基因频率明显低于对照组，ＲＲ值为０１３，Ｐ＜０．０５。结论：中国湖北汉族群体中ＭＧ与ＨＬＡ－ＤＲＢ１０２０２、等位基因正关联，而与ＨＬＡ－ＤＰＢ１０２０２等位基因呈负关联，与白种人群相比，这种关联对中国湖北汉族ＭＧ病人来说是特有的。

AIM:To investigate the association of polymorphism of HLA class Ⅱ genes (DRB1/DQB1/DPB1) with myasthenia gravies(MG).METHODS:HLA class Ⅱ alleles of 91 patients with MG and that of 168 normal individuals of Han nationality of Hubei province were typed by using PCR/SSP and PCR/RFLP.RESULTS:(1) Gene frequencies of HLA-DRB10901(RR=4.12, P <0.01), HLA-DQB10303(RR=3.04, P <0.01) and DPB1 0501(RR=1 22, P <0.01) are much higher in MG patients than in normal controls; (2) Gene frequencies of HLA-DPB10202(RR=0.12, P <0.05) are much lower in MG patients than in normal controls. CONCLUSION:There is a positive correlation between HLA-DRB10901 DQB1 0303 DPB10501 and MG. There is a negative correlation between HLA-DPB10202 and MG. These correlations are peculiar to MG patients of the Han nationality of Hubei province, as compared with Caucasians.