黄芪对大鼠心肌梗塞后左室功能及胶原改建的影响

The effect of Huang Qi injection on cardiac function and left ventricular collagen remodeling after myocardial infaction in rats

目的：在实验性大鼠心肌梗塞模型上、观察黄芪对心梗后左室功能及胶原改建影响。方法：心梗后腹腔注射黄芪注射液２或６周，用放免法测定血浆、心肌血管紧张素Ⅱ（ＡｎｇⅡ）及血浆醛固酮（ＡＬＤ）；动脉插管测定心功能；氯胺Ｔ法测定非梗塞区（含梗塞周边区）左室心肌羟脯氨酸的水平；免疫组织化学法测量非梗塞区左室心肌Ⅰ型及Ⅲ型胶原蛋白的比值。结果：与心肌梗塞对照组比较，黄芪可降低６周时的血浆、非梗塞区心肌ＡｎｇⅡ及血浆ＡＬＤ水平（Ｐ＜００５）；２周及６周时可明显升高＋ｄｐ／ｄｔｍａｘ及－ｄｐ／ｄｔｍａｘ水平（Ｐ＜００５）；减少非梗塞区左室心肌羟脯氨酸的含量；降低非梗塞区左室心肌Ⅰ型及Ⅲ型胶原蛋白的比值（Ｐ＜００５）。结论：黄芪对心梗后左室功能及胶原改建可产生有益的影响。

AIM:To observe the effect of Huang Qi injection on left ventricular function and collagen remodeling of noninfarcted area myocardial infarction.Prolonged inotropic stimulation with digoxin during healing after myocardial infarction increased infarct bulging. Huang Qi is an inotropic durg, but its effect on left ventricular remodeling is unknown. METHODS: Ten sham operated SD rats served as controls (sham). Eighty-eight rats with a moderate to large myocardial infarction were randomly divided into eight groups. The rats were treated with Huang Qi injection (2 mg/d intraperitoneal injection) and/or captopril (2g/L drinking water).Acute myocardial infarction(AMI) group did not receive drug treatment. The animals were sacrificed at 2 weeks or 6 weeks after coronary artery ligation. The levels of plasma and myocardial angiotensin Ⅱ and plasma aldosterone (by RIA) and left ventricle function (by catheter measuring) were studied at different time. The collagen content(hydroxyproline levels) and the ratio of type Ⅰand Ⅲ collagen (by immunohistochemical staining) of noninfarcted area were also assessed in all rats at the second or the sixth week.RESULTS:Compared with AMI groups, both Huang Qi and captopril decreased the levels of plasma and myocardium angiotensin Ⅱ and plasma aldosterone at the sixth week ( P<0.05 ) and Huang Qi improved systolic and diastolic function ( P<0.05 ).Compared with sham group,both collagen content (hydroxyproline levels) and the ratio of type Ⅰ/Ⅲ collagen of noninfarcted area (including infarct border) increased in AMI2 and AMI6 group ( P<0.05 ).The hydroxyproline levels and the ratio of type Ⅰ/Ⅲ collagen significantly decreased after Huang Qi and/or captopril treatment compared with AMI group at the second or the sixth week( P <0.05).CONCLUSION:Huang Qi can improve cardiac function after myocardial infarction and has beneficial effect on left ventricular collagen remodeling.