生长抑素对羟自由基损伤的离体人胃粘膜细胞的作用

Effects of somatostatin on isolated human gastric mucosal cells injured by hydroxyl radicals

目的和方法：利用人手术切除胃标本，应用Ｐｒｏｎａｓｅ－ＥＤＴＡ法进行人正常胃粘膜细胞的分离及孵育，并采用羟自由基建立细胞损伤模型，观察八肽生长抑素（ＳＳ－８）对细胞损伤的影响。结果：（１）采用Ｐｒｏｎａｓｅ－ＥＤＴＡ法分离人胃粘膜上皮细胞，每４ｃｍ×５ｃｍ大小胃粘膜可分离细胞数为２４×１０６个，细胞存活率达９０％以上。（２）Ｆｅ２＋－Ｈ２Ｏ２体系产生的羟自由基（ＯＨ·）可直接导致人胃粘膜细胞存活率下降，细胞乳酸脱氢酶漏出量及细胞丙二醛含量增加，以ＳＳ－８预处理可减轻上述改变。结论：ＳＳ－８可减轻ＯＨ·对孵育状态下人胃粘膜细胞的损伤，作用与其减轻细胞的脂质过氧化程度有关。

AIM and METHODS:Pronase-EDTA method was applied to human gastric specimen to dissociate and incubate gastric mucosal cells. A model of hydroxyl-induced human gastric mucosal cell injury was established and effects of sandostatin(SS-8) on the injured cells were observed. RESULTS:(1)2.4×10 6 cell could be dissociated from per gastric mucosa sized 4 cm×5 cm, the cells mainly consisted of parietal cells, chief cells and mucous neck cells, the cell viability was over 90%.(2)Hydroxyl radical (OH·) generated by Fe 2+ -H 2O 2 system could directly induce a decrease of cell viability and an increase of cell lactodehydrogenase (LDH) leakage, and cellular malondialdehyde (MDA) content was also augmented. These changes could be partially reversed by pretreatment of SS-8. CONCLUSION:SS-8 could inhibit cellular MDA increase, thus lessen OH· induced injury to gastric mucosal cells.