siRNA-TRF2对人乳腺癌MCF-7细胞增殖的影响

Effects of siRNA-TRF2 on Cell Proliferation of Human Breast Cancer Cell Line MCF-7

目的构建表达端粒重复序列结合因子2基因小干扰RNA的腺病毒表达载体(rAd-shR-NA-TRF2),探讨其对人乳腺癌MCF-7细胞增殖的影响。方法根据预实验筛选出的针对人TRF2 mRNA的特异性siRNA靶序列,构建表达siRNA-TRF2的重组腺病毒载体rAd-shRNA-TRF2,转染人乳腺癌MCF-7细胞后,用MTT比色法检测MCF-7细胞增殖情况,流式细胞仪检测细胞周期分布情况。结果 rAd-shRNA-TRF2转染MCF-7细胞后,MCF-7细胞增殖抑制、细胞周期阻滞于G0/G1期、增殖指数显著下降。结论通过RNAi抑制TRF2基因表达进而抑制人乳腺癌MCF-7细胞增殖的方法有望成为肿瘤基因治疗的一个新策略。

Objective Constructed recombinant adenovirus vector for the express of small interfering RNA targeting telomeric repeatbinding factor2,i.e.rAd-shRNA-TRF2 and to investi gate the effects of rAd-shRNA-TRF2 on cell proliferation of human breast cancer MCF-7 cells.Methods Constructed recombinant adenovirus vector（rAd-shRNA-TRF2）expressing siRNA-TRF2 was constructed by selecting,siRNA witch can specifically and effectively express TRF2 mRNA based on preliminary experiments.After rAd-shRNA-TRF2 was transfected into MCF-7 cells,the cell growth were detected by MTT assay and the cell cycle was observed by means of flow cytometry.Results The cell proliferation of MCF-7 was inhibited.The cell cycle of MCF-7 was at G0/G1phase and the cell proliferation index（PI）was breakdown significantly after rAd-shRNA-TRF2 was transfected into MCF-7 cells.Conclusions The cell proliferation of MCF-7 is prohibited by inhibiting the TRF2 express,which maybe become a new gene therapy strategy for the tumor treatment.