裸小鼠人宫颈癌组织模型的建立及其生物学特性初探

Establishment of Human Cervical Cancer Model in Nude Mice and Preliminary Study of Its Biological Features

目的建立人宫颈癌HeLa细胞在裸小鼠的体表肿瘤模型,并研究其成瘤性及生物学特性。方法以不同浓度人宫颈癌HeLa细胞悬液在裸小鼠皮下接种建立人宫颈癌裸小鼠模型,观察荷瘤鼠的成瘤率及肿瘤的一般特性,病理检查其组织学特性,流式细胞术检测其生长、凋亡情况,免疫组织化学法检测肿瘤组织中Survivin,Caspase-3和JAK-STAT3蛋白的表达情况。结果成瘤率为100%,肿瘤生长以局部浸润为主,未见转移瘤,所有新生肿瘤组织符合人宫颈癌细胞组织特点,绝大多数肿瘤细胞处在增殖分化期,标本宫颈癌组织中有Survivin,Caspase-3和STAT3蛋白的阳性表达。结论成功建立了裸小鼠人宫颈癌组织模型,该模型较好地模拟了人宫颈癌的生物学特性,为进一步研究针对诱导细胞凋亡治疗的新方法提供了理想的动物模型。初步观察了不同浓度HeLa细胞悬液接种生长的肿瘤组织在生长方面的区别。

Objective To establish the human cervical cancer model in nude mice and to investigate its biological features.Methods Human cervical cancer HeLa cells suspension with different concentration was injected into the subcutaneous to establish the human cervical cancer model in nude mice.Success rate of tumor transplantation,general characteristic,tumor growth,metastasis and histological features were observed.The cell growth and apoptosis in tumor tissue were estimated by Flow Cytometry（FCM）.The express of the proteins such as Survivin,Caspase-3 and STAT3 in the tumor tissue was measured by the immunohistochemical（IHC）method.Results The tumorigenicity rate is 100%.The transplanted tumors grew well by local invasion without metastasis.The histological appearance of the tumor cells before and after transplantation was very similar.FCM results showed most of the tumor cells are in the proliferation and differentiation period.The xpression of the proteins Survivin,Caspase-3 and STAT3in the tumor tissue were positive measured by IHC.Conclusions Human cervical cancer model in nude mice has been successfully established.Pathological examination,FCM and IHC method for Survivin,Caspase-3 and STAT3 proved this animal model has simulated the biological features of human cervical cancer.Our work produced an ideal model for the mechanism research of apoptosis-inducing therapy.The differences in cell growth status from the HeLa cells with different cell suspension concentrations was preliminarily observed.