结肠癌患者外周血血管紧张素转换酶基因多态性

Polymorphism of Angiotensin Ⅰ-converting Enzyme Gene in Peripheral Blood of Colon Cancer Patients

目的研究血管紧张素转换酶(ACE)基因多态性与结肠癌的关系。方法采用聚合酶链反应(PCR)检测30例结肠癌患者和30例正常人外周血中ACE基因缺失型(DD型)、插入型(ID型)和Ⅱ型及I/D等位基因在结肠癌患者外周血中的分布规律。结果结肠癌患者外周血中存在ACE基因插入/缺失(I/D)多态性,DD型、ID型和Ⅱ型3种基因型的占比分别为6.67%,36.67%,56.66%,I/D等位基因分布频率分别为93.33%和6.67%,健康对照组DD型、ID型和Ⅱ型3种基因型的占比分别为16.67%,53.33%,30.00%,I/D等位基因分布频率分别为83.33%和16.67%,经统计学分析,两组间DD型、ID型和Ⅱ型的分布频率差异有统计学意义(P<0.05),I/D等位基因分布频率差异无统计学意义(P>0.05)。结论结肠癌患者外周血中存在ACE基因I/D多态性,Ⅱ型基因分布较健康对照组显著升高,预示与结肠癌发病存在一定关系。

Objective To investigate the correlation between the insertion/deletion（I/D）polymorphism of angiotensinⅠ-converting enzyme gene（ACE）and cancer of colon.Methods Polymerase chain reaction（PCR）was served to detect insertion/deletion genotypes and I/D alleles of ACE gene in peripheral blood of 30 colon cancer patients,30 normal persons as control.Results In colon cancer patients,the frequencies of genotypes of DD,ID andⅡ of ACE gene were 6.67%,36.67% and 56.66%,and I/D alleles were 93.33%and 6.67%,respectively.In contrast,the frequencies of genotypes of DD,ID andⅡof ACE gene were 16.67%,53.33%and 30.00%and I/D alleles were 83.33%and16.67%respectively in normal control.The distribution of DD,ID andⅡgenotypes of ACE gene between two groups was significant（P〈0.05）.On the contrary,I/D alleles between two groups was not significant（P〉0.05）.Conclusions There was an I/D polymorphism of ACE gene in colon cancer patients,and the distribution of II genotype were markedly higher than that in the control,which might be correlated to the prevalence of colon cancer.