基因工程IFNα_(2a)对肿瘤细胞生长的抑制作用

The Study on the Anti-Cox B_3 Virus Effect of Recombinant Interferon-a_(2a) in Wish Cell lines

采用α－干扰素抑制各种肺癌细胞生长的MTT法。结果：不同品牌的10000，1000，100IU／ml的IFNα2a对人脑胶质瘤细胞（SHG—44）、人骨肉瘤细胞（HOS－8603）、人白血病细胞（HL－60）、红白血病细胞（K562）和淋巴瘤细胞（Raji）的生长抑制率分别为20％，10％，5％左右。结论：进口和国产的不同品牌IFNα2a。对上述5种肿瘤细胞株均有较明显的生长抑制效应，且抑制率相近。

In the present report we used the method of microdose cytopathogenic inhibition effect assay to study the anti-CoxB3 virus effect of recornbinant intefferon-a2a in Wish cell lines. The results indicated that both Wandferon and Roferon -A exhibited antiviral effect obviously. In our experiment 500IU/ml, 50IU/ml and 5IU/ml of IFN - a2a can preventWish cells from idecting by CoxB3 virus of 3 log4, 2 log4 and 1 log4 TCID50 respectively. The results als0 suggested thatthere were no apparent differeces between Wandferon and Rofemo-A.