FISH技术检测宫颈病变中hTERC基因的表达及意义

Expression and significance of hTERC gene by FISH detection in cervical lesions

目的:检测宫颈癌和宫颈上皮内瘤变中的hTERC基因的扩增情况。方法:采用双色荧光原位杂交技术(FISH)检测100例宫颈病变脱落细胞中的hTERC基因表达。结果:良性细胞学病变、不典型鳞状细胞(ASC)、低度鳞状上皮内瘤变(LSIL)、高度鳞状上皮内瘤变(HSIL)脱落细胞hTERC基因阳性表达率分别为9.09%、25.00%、54.29%、91.67%。慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈癌脱落细胞hTERC基因阳性表达率分别为6.67%、14.29%、64.71%、75.00%、90.24%。结论:慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ到宫颈癌的hTERC基因阳性表达率逐渐增高,宫颈低度病变与高度病变hTERC基因检测可成为临床检查宫颈癌的新手段。

Objective： To detect the human telomerase（hTERC）amplication of cervical carcinoma and CIN.Methods： hTERC expression were detected by FISH from 100 cases cervical intraepithelial neoplasia.Results： Rate of expression of hTERC in chronic cervicitis,atypical squamous cells（ASC）,low-grade squamous intraepithelial lesion（LSIL）,high-grade squamous intraepithelial lesion（HSIL） were 9.09%,25.00%,54.29%,and 91.67%, respectively. Expression rate of hTERC in chronic cercicitis,CINⅠ,CINⅡ,CINⅢ and cervical cancer were 6.67%,14.29%,64.71%,75.00%,and 90.24%,respectively.Conclusion： The positive of hTERC expression is closely related to cervical disease level.FISH may be an adjunct to cytology screening, especially high-risk patients.