乳糖操纵子介导的半乳糖苷酶在转基因动物中的应用前景

Prospect of the Lac Operon Mediated Galactosidase Applied in Transgenic Animals

细菌的乳糖操纵子可以在哺乳动物中调控基因的表达,修饰阻抑物基因和操纵基因可调控阻抑物诱导能力及其对操纵基因的亲和力,更好的适应高等动物的内环境。半乳糖苷酶对乳糖操纵子系统有正向调控作用,利用乳糖操纵子在转基因动物中可诱导性调控半乳糖苷酶基因的表达,能有效的提高转基因动物利用半乳糖苷、吸收营养物质的能力。以下从乳糖操纵子的结构功能、乳糖阻抑物功能活性的基因调控、操纵基因的功能以及其在哺乳动物的应用现状四个方面,结合半乳糖苷酶的生理功能和其在转基因动物中应用两个方面进行综述,对乳糖操纵子介导的半乳糖苷酶在转基因动物中的应用效果和前景进行了分析探讨。

Bacterial lac operon can regulate gene expression in mammalian.Modified lac operon alter the inducibility and affinity binding to lac operator,which will be better to adapt higher animals＇ internal environment.Galactosidase has a positive regulation effect on the lac operon system.Lac operon regulate galactosidase inducible expression can improve transgenic animals＇ ability of making full use of galactoside.Following from the structure and function of lac operon,the gene regulation properties,combined with galactosidase＇s physiological functions and applied in transgenic animals are reviewed.Analysis prospect of the lac operon mediated galactosidase applied in transgenic animals.