脱氢酶生物传感器研究关键技术与进展

Key Technologies and Progress of Amperometric Biosensors Based on Dehydrogenases

自然界中依赖烟酰胺类辅酶(NAD+或NADP+)的脱氢酶是氧化还原酶中最重要的一类,基于此类酶的生物传感器应用前景广阔,近年来发展迅速。构建这类传感器需要两项关键技术,即氧化型辅酶在电极表面的再生和辅酶固定化。介绍了辅酶电化学再生的主要方法、辅酶固定化的常见手段,以及相关的研究进展。

Dehydrogenases,which use nicotinamide adenine dinucleotide （NAD＋） or nicotinamide adenine dinucleotide phosphate （NADP＋） as the coenzyme for their catalyzed reactions,are the most important grou Pof oxidoreductases in the nature.In recent years,Biosensors based on NAD（P）＋-dependent dehydrogenases have been developed rapidly.Two key technologies are quite important for the construction of these biosensors.One is the electrochemical regeneration of oxidated form of coenzyme,and another is the coenzyme immobilization in electrode surface.Methods were reviewal to aid the electrochemical regeneration of coenzyme.Common ways of coenzyme immobilization were also introduced.In addition,research progress of the two key technologies was reviewed.