重组人促红细胞生成素对腹部开放伤海水浸泡大鼠的影响

Effects of recombinant human erythropoietin on inflammatory response induced by open abdominal wounds following artificial seawater immersion in rats

目的 探讨重组人促红细胞生成素（EPO）对腹部开放伤海水浸泡大鼠炎症反应的调节作用.方法 把实验用清洁级健康雄性Wistar大鼠随机分成4组：EPO预处理组、观察组、小剂量EPO救治组、大剂量EPO救治组,每组15只,制备腹部开放伤加人工海水浸泡致急性应激性炎症损伤动物模型,观察各组实验动物的肌酸激酶、肌酸激酶同工酶、肿瘤坏死因子α（TNF-α）、白介素6（IL-6）、炎症介质C3a、高敏C反应蛋白、血清免疫球蛋白IgA,比较这些指标在预处理组与观察组、大小剂量救治组之间的差异.结果 各组Wistar大鼠在腹部开放伤加海水浸泡3 h后均发生了急性炎症反应.EPO预处理组与观察组比较,肌酸激酶、肌酸激酶同工酶、TNF-α、IL-6、炎症介质C3α、高敏C反应蛋白、血清免疫球蛋白IgA等指标差异均有统计学意义（P〈0.05）;而大、小剂量EPO救治组间各项指标差异无统计学意义（P〉0.05）.结论 EPO具有减轻机体炎症反应、降低腹部开放伤海水浸泡大鼠炎症损伤的作用.

Objective To explore the role of recombinant human erythropoietin （EPO） in the regulation of inflammatory response induced by open abdominal wound coupled with artificial seawater immersion in rats. Methods Male healthy Wistar rats of clean grade were randomly divided into 4 groups （n = 15）： the EPO pretreatment group, the control group, the low-dose EPO treatment group and the highdose EPO treatment group. An animal model of acute stress - induced inflammatory lesion was developed by open abdominal wounds coupled with artificial seawater immersion. Concentrations of creatine kinase, creatine kinase isoenzyme, TNF-α, IL-6, inflammatory mediators C3α, serum high- sensitivity C-reactive protein and serum immunoglobulin IgA were monitored. And comparisons were made for the differences in those observed indicators between the EPO pretreatment group, the control group, the low and high dose EPO treatment groups. Results Acute inflammatory responses induced by open abdominal wounds coupled with 3-h seawater immersion were observed in rats of each group. Statistical differences could be seen in creatine kinase, creatine kinase isoenzyme, TNF-α, IL-6, inflammatory mediators C3a,serum high-sensitivity C-reactive protein, serum immunoglobulin IgA and other indicators between the EPO pretreatment group and the control group （P 〈0. 05）. No significant differences were noted in those variables between the high-dose EPO treatment group and the low - dose EPO treatment group （P 〉 0. 05）. Conclusions Erythropoietin （EPO） could regulate inflammatory responses and decrease inflammatory lesion induced by open abdominal wounds combined with seawater immersion in rats.