不同氧疗方式对急性一氧化碳中毒大鼠血气的影响

Effect of different oxygen therapy on blood gas in rats following acute carbon monoxide poisoning

目的 观察不同氧疗方式对急性一氧化碳中毒（acute carbon monoxide poisoning,ACOP）大鼠血气的影响.方法 将70只雄性Wistar大鼠随机分成健康对照组10只;染毒即刻组12只;余48只CO染毒后再分为4组,分别为空气组、鼻导管组、面罩组、HBO组各12只.制备ACOP动物模型,给予3种不同方式的氧疗,自腹主动脉取血行血气分析.结果 pH值：染毒即刻即出现明显下降（P〈0.01）,鼻导管及面罩吸氧后均低于正常（P〈0.05）,HBO治疗后接近正常（P〉0.05）.PaO2及PaCO2：染毒后各组差异无统计学意义（P〉0.05）.乳酸及COHb%：染毒即刻即出现明显升高（P〈0.01）,经鼻导管吸氧后高于正常（P〈0.05）;面罩及高压氧治疗后接近正常（P〉0.05）.HCO3-：染毒后各组均明显低于正常（P〈0.01）.结论 ACOP后不同氧疗方式均可改善ACOP缺氧和大鼠的代谢性酸中毒,在3种方式的氧疗中,HBO组清除大鼠体内COHb最显著.

Objective To observe the effect of different oxygen therapies on blood gas in rats following acute carbon monoxide poisoning. Methods Seventy male Wistar rats were randomly divided into the control group （n=10）, the COST group （sacrificed instantly after CO exposure） （n = 12）, and the remaining 48 animals were further divided into 4 groups of 12 animals each, i.e. the air breathing group; the nasal catheter oxygen breathing group, the face mask oxygen breathing group, and the hyperbaric oxygen exposure group. The last 4 groups were exposed to either normal air or oxygen for a duration of 100 minutes, following acute carbon monoxide poisoning. The animal model of acute carbon monoxide poisoning （ACOP） was developed as designed, and 3 different therapies were administered to the animals. Then, blood samples were taken from abdominal aorta for blood gas analysis. Results pH： pH value in the COST group was significantly lower than the control group （P 〈0.01）, and values in the nasal catheter oxygen breathing group were all lower than those of the control group（P 〈 0.05）, and the value measured in the HBO group was close to the control group（P 〉0.05）; PaO2 and PaCO2： no statistical significance in PaO2 or PaCO2 could be seen between the groups after ACOP（P 〉0.05）; Lactic acid and COHb%： levels of lactic acid and COHb% in the COST group increased significantly, when compared with those of the control group （P 〈0.01）, the value in the nasal catheter oxygen breathing group was higher than that in the control group（P 〈0.05）, and the values in the face mask oxygen breathing group and HBO group were close to those in the control group（P 〉0.05）; Bicarbonate （HCO3 -）：statistical differences in HCO3 - between the groups could be noted after ACOP （P 〈 0. 05）. Conclusions Various types of oxygen therapy could rectify hypoxia and metabolic acidosis in rats following ACOP. Compared with other two types of therapy, HBO could remove carboxyhemoglobin （COHb） from the body most significantly.