血透患者血清庚型肝炎病毒RNA及抗体检测

Detection of serum HGV RNA and HGV IgG antibody in hemodialysis patients

目的：了解血透患者庚型肝炎病毒（ＨＧＶ）感染率及与其他肝炎病毒重叠感染的情况。方法：收集５７份血透患者血清标本，根据ＨＧＶＲＮＡ保守片段５′非编码区序列，设计了两对引物，采用逆转录巢式ＰＣＲ扩增ＨＧＶＲＮＡ，以及ＥＩＡ法检测ＨＧＶＩｇＧ抗体。同时还检测了ＨＢＶＤＮＡ及ＨＣＶＩｇＧ抗体。结果：５７份标本中，４份ＨＧＶＲＮＡ阳性（７．０％），２份ＨＧＶＩｇＧ抗体阳性（３．５％）。ＨＧＶＲＮＡ与ＨＧＶＩｇＧ抗体呈“分离”现象。４份ＨＧＶＲＮＡ阳性标本中２份为ＨＢＶＤＮＡ阳性，２份为ＨＣＶＩｇＧ抗体阳性；其中１份标本ＨＢＶＤＮＡ和ＨＣＶＩｇＧ抗体均为阳性。结论：血透患者ＨＧＶ感染率高于健康献血员和正常人群，ＨＧＶ可与ＨＢＶ和ＨＣＶ重叠感染。

Objective: To determine the HGV infection rate and status of superinfection with other hepatitis virus in hemodialysis patients. Methods: Fifty seven serum samples were collected from hemodialysis patients. Two sets of primers were designed and synthesized according to the conserved region at 5 noncoding region of HGV RNA sequence. HGV RNA fragment was amplified by reverse transcriptnested PCR, and IgG antibody to HGV was tested by EIA. Meanwhile, HBV DNA and HCV IgG antibody were tested in all samples. Results: Among 57 serum samples detected, 4 samples were positive for HGV RNA(7.0%), and 2 were positive for HGV IgG antibody (3.5%).Among samples positive for HGV RNA, 2 were positive for HBV DNA, and 2 were positive for HCV IgG antibody, and one of which was positive for both HBV and HCV markers. Those samples positive for HGV RNA were negative for HGV IgG antibody and vice versa. Conclusion: The infection rate of HGV in hemodialysis patients may be higher than in healthy blood donors and heal Patients with HGV infection can be superinfected with HBV and HCV.