大蒜素诱导卵巢癌细胞株OVCA-3凋亡

Allicin induced apoptosis of human ovarian carcinoma cell line OVCA3

目的：探讨大蒜素诱导卵巢癌细胞株ＯＶＣＡ－３凋亡规律。方法：应用ＤＮＡ断裂点标记（ＴＵＮＥＬ）法、ＤＮＡ凝胶电泳以及光镜等技术观察有关细胞的凋亡情况。结果：０．０１～１００μｇ／ｍｌ的大蒜素作用ＯＶＣＡ－３细胞２ｈ及１０μｇ／ｍｌ的大蒜素作用ＯＶＣＡ－３细胞不同时间，锥虫蓝染色阳性率均在１８．５％以下。ＴＵＮＥＬ显示：１～１００μｇ／ｍｌ的大蒜素作用ＯＶＣＡ－３细胞２ｈ，即出现典型的细胞凋亡特征；５０μｇ／ｍｌ时凋亡率为６５．９％。结论：一定剂量范围内的大蒜素可在直接杀伤作用较小的情况下明显诱导ＯＶＣＡ－３细胞凋亡，不会因细胞坏死而引起炎症反应，这对于大蒜素应用于临床将有重要意义。

Objective: To investigate the rule of allicin induced apoptosis. Methods: Terminal deoxylnucleotide transferase directed XUTP nick and end labelling (TUNEL) assay, DNA gel electrophoresis and light microscopy were used to observe apoptosis. Results: Trypan Blue staining positive rates of OVCA3 cell were under 18.5% after being exposed to allicin at the dosage 0.01￣100 g/ml for 2 h and 10 g/ml for different time. Allicin induced apoptosis of OVCA3 cells at 1100 g/ml for 2 h. Apoptosis peak was shown at 50 g/ml. Apoptotic cells accounted for 65.9% of the population. Z]Conclusion: Allicin of appropriate dosage induces apoptosis of OVCA3 cell, while has little toxicity on OVCA3 cell. That is important for clinical application of allicin.