红景天保护缺血再灌注损伤鼠脑细胞的作用及机理研究

Study on protection of Rhodiola sachalinensis A.R on cerebral neurons against injury of the ischemia reperfusion in rats

目的研究红景天对大鼠脑缺血再灌注损伤的作用。方法４ＶＯ法复制缺血再灌注动物模型；放免法及化学发光法测定乙酰胆碱（Ａｃｈ）、一氧化氮（ＮＯ）及内皮素（ＥＴ）含量；细胞培养观察红景天对神经细胞的作用。结果（１）缺血再灌注组（ＩＲ）Ａｃｈ含量显著低于假手术组（ＳＡＭ），药物预防后缺血再灌注组（Ｒ＋ＩＲ）及缺血再灌注后药物治疗组（ＩＲ＋Ｒ）较ＩＲ组显著升高。（２）ＩＲ组ＮＯ含量显著高于ＳＡＭ组，Ｒ＋ＩＲ组及ＩＲ＋Ｒ组ＮＯ含量显著降低。（３）ＩＲ组ＥＴ含量显著高于ＳＡＭ组而Ｒ＋ＩＲ组显著降低。（４）红景天甙培养组细胞存活率及ＬＤＨ含量明显高于对照组，ＮＭＤＡ损伤＋红景天甙组细胞存活率明显高于ＮＭＤＡ损伤组，ＬＤＨ含量却明显降低。结论红景天对大鼠脑缺血再灌注损伤时脑神经细胞具有保护作用。

Objective To study the protection of Rhodiola sachalinensis A.R on cerebral neurons against the injury of ischemia reperfusion in rats. Method The experimental rats,which were readied with 4VO method ,were divided into 5 groups. The changes of Ach,NO and ET in brain tissue of different group rats were measured. Results (1) the contents of Ach of brain tissue of IR group rats were lower than that of SAM group ( P <0.01),but in R+IR group and IR+R group,contents of Ach were higher than that of IR group. (2) The contents of NO of IR group rats were higher than SAM group( P <0.01),and in R+IR and IR+R groups ,the contents of NO were lower than IR group ( P <0.05, P <0.01). (3)The contents of ET in IR group were higher than SAM group,but in R+IR group ,it was lower than IR group. (4)Compared by control group,the survival rate of cultured hippocampal neurons and the content of LDH in media in Rhodiola. Treated group were obviously higher,in NMDA+Rhodiola sachalinensis group,the survival rate was higher than NMDA group,content of LDH in media was lower than NMDA group. Conclusion The Rhodiola sachalinensis can protect the brain tissue of ischemia reperfusion rats.