过瘤胃保护性γ-氨基丁酸的调制与稳定性研究

Preparation and Stability of Rumen Protected γ-aminobutyric Acid

本试验旨在研制一种低瘤胃降解率和高瘤胃后释放率的过瘤胃γ-氨基丁酸(γ-aminobutyricacid,GABA)。采用L9(34)正交设计,以GABA含量、包被材料和压力为变量,筛选出保护率较高的样品,分别采用半体内法和体外法测定其瘤胃降解率和瘤胃后释放率。结果显示,固体棕榈油脂作为单一被材比混合被材具有更好的过瘤胃保护效果。当GABA含量50%、用固体棕榈油脂包被、压力11kPa下制得的样品瘤胃降解率显著低于其他样品,总利用率75.8%;GABA含量50%、固体棕榈油脂与单硬脂酸甘油酯等质量混合物包被、压力9kPa下制得的样品其瘤胃后释放率最高。利用固体棕榈油脂包被在适宜条件下可调制瘤胃降解率低、瘤胃后释放率高的过瘤胃保护GABA。

The study was conducted to develop a rumen protected γ-aminobutyric acid （GABA） with low ruminal degradability and high post-ruminal dissolution.GABA content,coating material and pressure were used as variables in a L9（34） orthogonal design to select samples with high insoluble rate,then ruminal degradability and post-ruminal dissolution were determined.The results showed as follows：as coating material,solid palm oil product was superior to the mixture of solid palm oil and glyceryl monostearate.The sample produced under the condition of 50% GABA,encapsulated with solid palm oil and under the pressure of 11 kPa was degraded at least in rumen.Its utilization efficiency was 75.8%.The sample produced under the conditions of GABA content of 50%,encapsulated with solid palm oil and glyceryl monostearate equal mass mixture and under the pressure of pressure 9 kPa presented the highest post-ruminal dissolution rate.It is suggested that the solid palm oil can be used as coating material to produced rumen protected GABA with low ruminal degradability and high post-ruminal dissolution.