摘要
采用原代培养的小鼠肝细胞,以3H-胸腺嘧啶(3H-TdR)和3H-亮氨酸(3H-Leucine)掺入的方法,研究齐墩果酸(OLA)预处理小鼠肝细胞DNA和蛋白质合成速率的影响。结果发现,经OLA预处理后的小鼠,其肝细胞DNA和蛋白质合成速率明显增加。提示OLA经体内代谢后,能提高肝细胞DNA和蛋白质的合成。
The methods of 3 H-TdR and 3 H-Leucine incorporation were used to observe the effects on the synthetic rate of DNA and protein in primary cultured hepatocytes of mice pretreated by OLA. After pretreating in vivo with OLA, the hepatocytes showed an obvious increase of synthetic rate of DNA and protein. The results suggest that OLA can promote the synthesis of DNA and protein in hepatocytes after being metabolized in the intact body.
出处
《贵阳医学院学报》
CAS
1999年第2期117-118,共2页
Journal of Guiyang Medical College
基金
中华医学基金会(CMB)奖学金资助
关键词
齐墩果酸
小鼠
DNA
肝疾病
蛋白质
OLA(3β-hydroxy-olea-12-en-28-oid acid)
liver
mice
DNA