HPLC法测定杠板归中槲皮素的含量

Determination of Quercetin in Polygoni Perfoliati Herba by HPLC

目的:建立草药杠板归中槲皮素含量的测定方法。方法:采用高效液相色谱法,色谱柱为AE.Lichrom C_(18)柱(150mm×4.6 mm,5μm),流动相为甲醇-0.4%磷酸(50:50),流速为1.0 ml·min^(-1),检测波长为360 nm。结果:槲皮素的进样量在0.012～0.616μg范围内与峰面积线形关系良好(r=0.999 9),平均回收率为98.64%(RSD=0.83%)。结论:此法操作简单,结果可靠,可用于杠板归的质量控制。

Objective： To establish an HPLC method for content determination of quereetin from polygoni perfoliati herba. Method： The AE. Lichrom C 18 column （ 150 mm × 4. 6 mm,5 μm）was used, the mobile phase was the mixture of methanol and 0. 4% phosphoric acid solution at a flow rate of 1.0 ml·min^-1. The wavelength was 360 nm. Result： The calibration curve showed good linearity within the range of 0. 012-0. 616 μg （ r = 0. 999 9）, the average recovery was 98. 64% （ RSD = 0. 83% ）. Conclusion： The method is simple, convenient, accurate, and can be used to control the quality of polygoni perfoliati herba.