微束等离子喷涂羟基磷灰石涂层的细胞生物相容性研究

Biocompatibility of cultured osteoblasts on microplasma sprayed hydroxyapatite caoting

目的微束等离子喷涂由于喷涂功率低,喷涂温度低,可以解决等离子喷涂羟基磷灰石分解这一问题。因此,本研究以大气等离子喷涂羟基磷灰石涂层(APS-HA)为对照组,微束等离子喷涂羟基磷灰石涂层(MPS-HA)为实验组,研究微束等离子喷涂羟基磷灰石涂层(MPS-HA)的细胞生物相容性。方法以MC3T3-E1小鼠成骨细胞为实验细胞,将成骨细胞接种到两种喷涂方法的生物材料表面,采用扫描电镜(SEM)、四唑盐比色法(MTT)、碱性磷酸酶检测(ALP),研究成骨细胞在两种生物材料表面形态、粘附、增殖、分化的影响。结果 APS-HA组和MPS-HA组均具有良好的生物相容性,MPS-HA组更有利于成骨细胞的生长、粘附、增殖,能促进成骨细胞向成熟的表型分化。结论微束等离子喷涂羟基磷灰石涂层(MPS-HA)与成骨细胞具有良好的相容性,材料表面有利于成骨细胞的粘附和增殖。为其成为新型口腔种植体提供理论依据。

Objective As microplasma sprayed hydroxyapatite （MPS-HA） is of low power and temperature, it might be used to solve the problem of atmospheric plasma sprayed hydroxyapatite coating（APS-HA） decomposition. The aim of this study was to evaluate the biocompatibility of cultured osteoblasts on microbeam plasma sprayed hydroxyapatite coating （MPS-HA）. Methods Mouse osteoblasts MC3T3-E1 cells were seeded on the surface of two sprayed biological materials： the atmospheric plasma sprayed hydroxyapatite coatings （APS-HA） as the control group, and microbeam plasma sprayed hydroxyapatite coating （MPS-HA） as the experimental group. The morphology, adhesion, proliferation and differentiation of osteoblasts growing on the two biological materials were examined by scanning electron microscopy （SEM）, MTT staining, and alkaline phosphatase （ALP） assay to compare their biocompatibility. Results Both the APS-HA and MPS-HA groups had good biocompatibility. However, the MPS-HA group was better in the cell growth, adhesion and proliferation, and could promote the osteoblast differentiation to mature phenotype. Conclusion Microplasma sprayed hydroxyapatite （MPS-HA） and osteoblasts have good biocompatibility and its surface is better for osteoblast adhesion and proliferation. Therefore, it provides a theoretical basis for development of a new oral implants.