甲氧氟烷吸入麻醉诱导中枢神经系统c-fos基因的表达

Cfos Expression within Central Nervous System of the Rat during Methoxyflurane Anesthesia

目的：了解甲氧氟烷吸入麻醉在中枢神经系统的作用部位。方法：应用免疫组织化学方法，光镜观察Ｆｏｓ蛋白阳性神经元在中枢神经系统的分布并计数。结果：大鼠吸入甲氧氟烷浓度为０７５％、１５％和２０％麻醉１小时后，Ｆｏｓ阳性神经元主要分布于杏仁核簇、弓状核（Ａｒｃ）、丘脑腹前核（ＡＶ）、终纹床核（ＢＳＴ）、中央灰质（ＰＡＧ）、背内侧梨状核（ＤＥｎ）、下丘脑背内侧核（Ｄｍ）、ＥＷ核（ＥＷ）、脚间核（ＩＰ）、内外侧僵核（Ｈｂ）、外侧隔核（Ｌｓ）、下丘脑室旁核腹侧部（Ｐａｖ）、丘脑室旁核（Ｐｖ）、丘脑再连合核（Ｒｅ）、菱形丘脑核（Ｒｈ）、丘脑中央中核（Ｃｍ）、视上核（Ｓｏ）和丘脑腹后外侧核（ＶＰＬ）。同时发现Ｆｏｓ阳性神经元的数目随着甲氧氟烷吸入浓度增加而增多。结论：研究表明Ｆｏｓ阳性神经核团参与了甲氧氟烷的麻醉过程，但对其核团之间纤维联系方式和相互作用机制仍需进一步研究。

Objective: To study the distribution of cfos oncogene expression within central nervous system(CNS) of the rat during methoxyflurane anesthesia. Methods:Cfos oncogene immunohistochemical technique (ABC method) was employed. Results:When methoxyflurane concentration was 075%,15% and 20%, most of Foslike immunoreactive neurons(FLN) appeared in arcuate hypothalamic nucleus(Arc), anteroventral thalamic nucleus(AV), amydaloid nucleus cluster, bednucleus of the stria terminalis(BST), midbrain periagueductal gray(PAG), dorsal endopiriform mucleus(DEn), dorsomedial hypothalamic nucleus(Dm), EdingerWestphal nucleus(EW), Interpeduncular nucleus(IP), medial andlateral habenular nucleus(Hb), lateral septal nucleus(LS), paraventricular hypothalamic nucleus(ventral part)(Pav), paraventricular thalamic nucleus(Pv), reuniens thalamic nucleus(Re), rhomboid thalamic nucleus(Rh), supraoptic nucleus(So) and ventral posterolateral thalamic nucleus(VPL) in the brain. In the experiment, we discovered yet that the member of FLN was adding steadlly along with increased the concentration of methoxyflurane. Conclusion:The results indicate that FLNs participate in the process of methoxyflurane anesthesia, but its mechanism needs to be studied in the future.