独活寄生汤结合骨髓基质干细胞复合体修复兔膝关节软骨缺损

Duhuojisheng decoction combined with bone marrow stromal stem cells complex in repair of articular cartilage defects in rabbits

背景:实验表明,骨髓基质干细胞复合脱细胞真皮基质能修复家兔膝关节软骨缺损,中药独活寄生汤能促进软骨的修复,且提高修复质量。目的:进一步验证独活寄生汤对骨髓基质干细胞复合体修复兔关节软骨缺损的影响,评价修复效果。方法:取兔骨髓基质干细胞进行体外增殖后,复合于改建后的脱细胞真皮基质载体上,植入到兔膝关节软骨缺损,并以中药独活寄生汤灌胃为独活寄生汤+干细胞组,并设立独活寄生汤组、单纯复合体组、空白组为对照组。于4,8,12周后分别对修复组织进行形态学观察及组织学检查。结果与结论:术后12周,独活寄生汤+干细胞组的缺损由透明软骨样组织充填,软骨及软骨下骨组织基本修复,修复的软骨组织在组织形态学上优于其他各组。结果证实,骨髓基质干细胞复合体能修复家兔膝关节软骨缺损,中药独活寄生汤能促进软骨的修复,且提高修复质量。

BACKGROUND：Bone marrow stromal stem cells （BMSCs） combined with acellular dermal matrix （ADM） can repair the articular cartilage defects in rabbits. Chinese medicine Duhuojisheng soup can promote cartilage repair,and improve the quality of repair. OBJECTIVE：To further verify Duhuojisheng decoction effects on the repair of rabbit articular cartilage defects using BMSCs complex. METHODS：Rabbit BMSCs following in vitro proliferation were compounded on ADM,which was then implanted into rabbit knee cartilage defects. Traditional Chinese medicine Duhuojisheng decoction was given,which served as a Duhuojisheng decoction ＋ stem cell group. This study also set Duhuojisheng decoction group,composite grouped and blank group （control group）. Morphological observation and histological examination were performed in repaired tissue at 4,8,12 weeks. RESULTS AND CONCLUSION：After 12 weeks,the defect in the Duhuojisheng detection ＋ stem cell group was filled with hyaline cartilage-like tissue; cartilage and subchondral bone tissue were basically repaired; repair of cartilage in histomorphology was superior to other groups. Results have indicated that BMSCs complex can repair articular cartilage defects in rabbits,and Chinese medicine Duhuojisheng decoction can promote cartilage repair,and improve the quality of repair.