蜂蜜治疗糖尿病创面与内皮祖细胞的动员

Honey for treatment of diabetic wounds and mobilization of endothelial precursor cells

背景:研究表明溃疡愈合的重要过程之一为溃疡的基质中形成新生血管,而骨髓来源的内皮祖细胞在这一过程中起到重要作用。骨髓来源的内皮祖细胞由骨髓向外周血动员以及其向溃疡处移行将有助于在溃疡处形成新生血管从而促进溃疡愈合,基质细胞衍生因子1α正是循环中调节血管生成前体细胞向受损组织移行的关键分子。目的:观察蜂蜜治疗后内皮祖细胞动员情况以及对基质细胞衍生因子1α表达的影响。方法:在链脲佐菌素介导的糖尿病大鼠模型的背部制造一个直径1.8cm的全层皮肤创面,随机分成蜂蜜组和生理盐水组,观察两组的创面变化。流式细胞仪测定大鼠外周血中内皮祖细胞的数量。实时定量PCR测定局部组织中基质细胞衍生因子1α的表达。结果与结论:在伤后3,7d蜂蜜组创面愈合面积比生理盐水组明显增加(P<0.05);创面模型建立后3d,外周血内皮祖细胞明显增加(P<0.05);实时定量PCR检测创面组织中基质细胞衍生因子1αmRNA的表达与生理盐水组相比较明显增强。结果提示蜂蜜可能通过基质细胞衍生因子1α的生成促进内皮祖细胞的动员,进而参与新生血管生成,促进糖尿病大鼠创面愈合。

BACKGROUND：New vessel is a basic process of wound healing,and bone marrow-derived endothelial progenitor cells （BM-EPCs） play a key role in this condition. It can contribute to form new vessels in ulcer area that BM-EPCs mobilize from bone marrow to peripheral blood circulation and migrate to ulcer wound. Stromal cell-derived factor-1α （SDF-1α） is on e key molecule,which regulates this migration of the precursor cells of new vessels to damaged tissue. OBJECTIVE：To investigate mobilization of endothelial progenitor cells following honey treatment and the effects of honey on SDF-1α expression. METHODS：A round full-thickness skin wound of 1.8 cm diameter was created on the backs of streptozotocin （STZ） induced diabetic rats. The rats were randomized into two groups：saline group and honey group. The wound surface was observed. The number of endothelial progenitor cells in rat peripheral blood was assayed using flow cytometry. Expression of SDF-1α was evaluated by real-time polymerase chain reaction. RESULTS AND CONCLUSION：The healing area of wound surface was significantly increased in the honey group compared with saline group at 3 and 7 days （P 0.05）. The number of endothelial progenitor cells in peripheral blood was significantly increased at 3 days （P 0.05）. Real-time polymerase chain reaction demonstrated that SDF-1α m RNAexpression was significantly higher in the honey group compared with saline group. Results suggest that honey could accelerate mobilization of endothelial progenitor cells by production of SDF-1α,participate in neovascularization,and promote wound healing in diabetic rats