血管组织工程种子细胞在再生医学中的应用

Application of vascular tissue engineered seed cells to regeneration medicine

背景:小口径人工血管替代人体小动脉和静脉一直未获得满意的效果,因此研制出一种拥有较高远期通畅率的小口径人工血管成为了一个重要的研究课题。目的:综述种子细胞在血管组织工程的研究进展。方法:以"Vascular tissue engineering,Seeding cells"为检索词,应用计算机检索Pubmed数据库1960/2009有关文章。纳入有关血管组织工程种子细胞的文献。排除原始文献设计方法简单、结果可靠性差、非英文文献及结果重复的文献,保留35篇文献做进一步分析。结果与结论:内皮细胞和平滑肌细胞是目前常用的种子细胞。内皮细胞和平滑肌细胞共同培养的体系,模拟体内环境,保持内皮细胞和平滑肌细胞具有正常的分泌功能和表型。骨髓间充质细胞可被有效的分离和扩增,在特定培养条件下可以诱导分化为多种血管细胞。在再生医学和生物组织工程方面有强大的潜力。

BACKGROUND：Small bore artificial vessels to substitute human body arteriole and vein have not obtained satisfactory outcomes. Thus,to develop a small bore artificial vessels with high long-term patency rate has been a key research program. OBJECTIVE：To review the latest progress of seed cells in vascular tissue engineering. METHODS：A computer based search was conducted in Pubmed database for articles concerning vascular tissue engineered seed cells published from 1960 to 2009 with the key words of ＂vascular tissue engineering,seeding cells＂. The literatures with simple design,poor reliability,non English or repetitive results were excluded. Finally,35 articles were included for further use. RESULTS AND CONCLUSION：The endothelial cells and smooth muscle cells were commonly used in the vascular tissue engineering. Endothelial cells and smooth muscle cells simulate in vivo environment to keep their normal secretory function and phenotype. Bone marrow mesenchyme stem cells can be effectively isolated and amplified,and can differentiate into various vascular cells under the special conditions and have strong potential in regeneration medicine and biological tissue engineering.