摘要
本文用多种有机溶剂对紫球藻中的类脂进行提取.利用气相色谱法对提取物中AA和EPA的含量进行了分析测定,并用光谱吸收法比较了提取物中脂溶性物质一色素的含量。结果表明,氯仿-甲醇和乙醇是两种有效的提取剂,提取效果较好:紫球藻细胞中类脂含量为8%~12%,并且国培养条件不同细胞中的类脂含量也有所不同。
Several organic extractants were used to separate the lipids in a kind of red microalga-Porphyridium cruentum,and the content of AA and EPA in the liplds were analyzed by the means of gas chromatograghy. The results showed that chloroform-methanol and alcohol were the effective extractants, and the big cells had much more lipids than the small cells.
出处
《中国海洋药物》
CAS
CSCD
1999年第2期28-31,共4页
Chinese Journal of Marine Drugs
基金
国家"九五"科技攻关!(96-C02-04-05)
华东理工大学生物反应器工程国家重点实验室资助项目。
关键词
紫球藻
花生四烯酸
二十碳五烯酸
色素
Porphyridium cruentum
arachidonic acid
eicosapentaenoic acid
pigment