海洋放线菌细胞毒抗肿瘤活性物质的初筛

SCREENING OF CYTOTOXIC ANTITUMOR AGENTS FROM MA- RINE ACTINOMYCETES BY MTT ASSAY

本文以改进的ＭＴＴ法为初筛模型，筛选海洋放线菌产生的细胞毒抗肿瘤活性物质。对４３４株海洋放线菌发酵液的筛选结果发现，约１６％的海洋放线菌发酵液对Ｐ_（３８８）和ＫＢ癌细胞的ＩＤ_（５０）等于或大于３２０，其中最高可达２０４８０。这表明海洋放线菌存在着活性高的细胞在抗肿瘤活性物质，是潜在的抗癌药源。

The modified MTT assay was used as in vitro model for screening potential cy- totoxic antitumor agents produced by marine actinomycetes. Tile cytotoxicity of fermentation broth from 134 strains of marine actinomycetes on P_(388) and KB cancer cells was evaluated and the result showed that about 16% of fermentation broths had ID_(50) at or above 320,of which the highest ID_(50) is 20480,indicating that marine actinomycetes,which produced antitumor agents with strong cytotoxicity,are the potential source of antitumor drugs.