摘要
本文以改进的MTT法为初筛模型,筛选海洋放线菌产生的细胞毒抗肿瘤活性物质。对434株海洋放线菌发酵液的筛选结果发现,约16%的海洋放线菌发酵液对P_(388)和KB癌细胞的ID_(50)等于或大于320,其中最高可达20480。这表明海洋放线菌存在着活性高的细胞在抗肿瘤活性物质,是潜在的抗癌药源。
The modified MTT assay was used as in vitro model for screening potential cy- totoxic antitumor agents produced by marine actinomycetes. Tile cytotoxicity of fermentation broth from 134 strains of marine actinomycetes on P_(388) and KB cancer cells was evaluated and the result showed that about 16% of fermentation broths had ID_(50) at or above 320,of which the highest ID_(50) is 20480,indicating that marine actinomycetes,which produced antitumor agents with strong cytotoxicity,are the potential source of antitumor drugs.
出处
《中国海洋药物》
CAS
CSCD
1999年第2期52-55,27,共5页
Chinese Journal of Marine Drugs
基金
国家自然科学基金资助项目!(编号:39670015)
国家教育部博士点基金项目!(编号:98038401)
关键词
海洋放线菌
抗肿瘤活性物质
体外筛选
marine actinomycetes: antitumor agents
MTT assay
cytotoxicity
in vitro screening