白介素-10-819 C/C和肿瘤坏死因子-α-1031 C/C/基因型与胃十二指肠疾病易感性的关系

Association between IL-10-819 C/C and TNF-α-1031 C/C genes and susceptibility of gastroduodenal diseases

目的分析人群中IL-10-819 C/C和TNF-α-1031 C/C基因型与胃十二指肠疾病的关系,确定携带以上该基因型的的人群罹患胃十二指肠疾病易感性的风险性。为临床诊断和预防这些疾病提供新的思路和方法。方法选取H.pylori阳性的48例慢性胃炎患者,46例十二指肠溃疡患者,51例胃溃疡患者,43例胃癌患者和100例健康对照者,2种基因型分别采用普通PCR和多重引物特异PCR法检测。结果在胃炎组中TNF-A-1031各基因型的频率(T/T,50%;T/C,40%;C/C,10%)与对照组(T/T,73%;T/C,25%;C/C,2%)比较,分布差异有统计学意义(χ2=9.975,P<0.05)。在胃溃疡组中TNF-A-1031各基因型的频率(T/T,49%;T/C,43%;C/C,8%)与对照组(T/T,73%;T/C,25%;C/C,2%)比较,分布差异有统计学意义(χ2=9.464,P<0.001)。在十二指肠溃疡组中TNF-A-1031各基因型的频率(T/T,72%;T/C,26%;C/C,2%)与对照组(T/T,73%;T/C,25%;C/C,2%)比较,分布差异有统计学意义(χ2=9.840,P<0.05)。在胃癌组中TNF-A-1031各基因型的频率(T/T,50%;T/C,41%;C/C,9%)与对照组(T/T,73%;T/C,25%;C/C,2%)比较,分布差异有统计学意义(χ2=9.335,P<0.001);Logistic回归分析与携带TNF-A-1031 T/T者比较,携带TNF-A-1031 C/C者发生胃炎的危险性为OR=7.60(95%CI:1.38-41.77);与携带TNF-A-1031 T/T者比较,携带TNF-A-1031 C/C者发生胃溃疡的危险性为OR=5.84(95%CI:1.00-33.84);与携带TNF-A-1031 T/T者比较,携带TNF-A-1031 C/C者发生十二指肠溃疡的危险性为OR=7.94(95%CI:1.44-43.67);与携带TNF-A-1031 T/T者比较,携带TNF-A-1031 C/C者发生胃癌的危险性为OR=6.95(95%CI:1.19-40.63)。在疾病组和对照组中IL-10-819的各基因型频率的分布差异无统计学意义(P>0.05)。结论 TNF-α-1031基因多态性与胃炎、胃溃疡、十二指肠、胃癌的易感性相关。

Objective To analyze the human IL-10-819 C/C and TNF-α-1031 C/C genes and their association with susceptibility of gastroduodenal diseases and provide new ideas and methods for clinical diagnosis and prevention of the disease.Method 51 H.pylori-positive patients with gastric ulcer,43 patients with gastric cancer and 100 healthy H.pylori positive controls were genotyped for IL-1B-31,TNF-A-308 and TNF-A-857 gene polymorphisms by the PCR-RFLP and ASP-PCR method.Statistical analysis of their polymorphism was performed.Result The frequencies of TNF-A-1031 genotypes in gastric ulcer group were significantly different from the control group（χ2=9.464,P〈0.001）,so were the frequencies of TNF-A-1031 genotypes in gastric cancer group（χ2=9.335,P〈0.001）.Multivariate logistic regression analysis showed that the subjects carrying TNF-A-1031 C/C genotype were at a 7-fold elevated risk for gastric ulcer,OR=7.60（95%CI： 1.38-41.77）,compared to those carrying TNF-A-3-1031 T/T;the subjects carrying TNF-A-1031 C/C genotype were at a 5-fold elevated risk for gastric ulcer OR= 5.84（95%CI： 1.00-33.84）,compared to those carrying TNF-A-3-1031 T/T.The subjects carrying TNF-A-1031 C/C genotype were at a 7-fold elevated risk for gastric ulcer,OR=7.94（95%CI： 1.44-43.67）,compared to those carrying TNF-A-1031 T/T;The subjects carrying TNF-A-1031 T/T genotype were at a 6-fold elevated risk for gastric cancer,OR=6.95（95%CI：1.19-40.63）compared to those carrying TNF-A-1031 C/C.IL-10-819 genotype distribution was not statistically significant in the disease group and the control group.Conclusion The polymorphism of TNF-α-1031 gene has close association with susceptibility of gastric ulcer and gastric cancer.