CyclinD1、CDK4、P16和结核菌L型感染在前列腺癌中的表达及临床意义

Expression and clinical significance of CyclinD1,CDK4,P16 and tuberculosis infection L-type in prostate carcinoma

目的探讨CyclinD1、CDK4和P16在前列腺癌中的表达以及结核菌L型感染率及临床意义。方法应用免疫组化和抗酸染色等方法检测了65例前列腺癌(carcinoma of prostate,PCa)和30例良性前列腺增生(benignprostatic hyperplasia,BPH)中的CyclinD1、CDK4和P16的表达,以及结核菌L型的检出率。并对前列腺肿瘤主要临床资料和病理分级参数进行比较,用χ2检验进行统计学处理。结果 CyclinD1、CDK4阳性表达前列腺癌明显高于前列腺增生(P<0.01);并与前列腺癌的临床分期、病理分级及淋巴结转移差异有统计学意义(P<0.01-0.05)呈正相关。P16阳性表达前列腺增生明显高于前列腺癌(P<0.01);与前列腺癌的临床分期、病理分级及淋巴结转移差异有统计学意义(P<0.01-0.05)呈负正相关。结核菌L型检出率前列腺癌明显高于前列腺增生;与前列腺癌的临床分期、病理分级及淋巴结转移差异有统计学意义(P<0.05)。结论 CyclinD1、CDK4和P16在前列腺肿瘤中不同程度异常表达以及结核菌L型检出率与肿瘤的临床分期、病理分级和转移相关,因此研究CyclinD1、CDK4和P16的阳性表达和结核菌L型感染与前列腺癌发生发展中可能有协同作用,具有重要的临床应用价值。

Objective To explore the expression of CyclinD1,CDK4 and P16 in prostate carcinoma as well as L-type Mycobacterium tuberculosis infection and its clinical significance.Method Immunohistochemistry,in situ hybridization and detection methods such as acid-fast stain were performed on 65 cases of prostate cancer（carcinoma of prostate,PCa） and 30 cases of benign prostatic hyperplasia（benign prostatic hyperplasia,BPH） for CyclinD1,CDK4 and P16 expressions,as well as the detection rate of TB L-type.The main clinical data and pathological classification parameters of prostate cancer were compared with χ^2 test for statistical analysis.Result The positive expression rates of CyclinD1 and CDK4 in prostate cancer were significantly higher than those in benign prostate hyperplasia（P〈0.01）;they were positively correlated with the clinical stage,histological grade and Lymph node metastasis of prostate carcinoma（P〈0.01-0.05）.The positive expression rate of P16 in benign prostate hyperplasia was significantly higher than that in prostate cancer（P〈0.01）;It was negative correlated with the clinical stage,histological grade and Lymph node metastasis of prostate carcinoma（P〈0.01-0.05）.The L-TB detecion rate of prostate cancer was significantly higher than that of benign prostate hyperplasia,with significant differences between clinical stages,histological grades and Lymph node metastasis（P〈0.05）.Conclusion The abnormal expression of CyclinD1,CDK4 and P16 in prostate tumors as well as the different levels of L-TB detection rate are correlated with the clinical tumor stage,grade and metastasis.Therefore,studies on the correlation between the positive expression of CyclinD1,CDK4 and P16 and tuberculosis L-type infection in the occurrence and development of prostate cancer can be valuable for clinical practice.