B7-H3在口腔鳞癌细胞中的表达及其意义

Expression of B7-H3 promotes cell proliferation in oral squamous cell carcinoma cell line

目的:研究B7-H3在口腔鳞癌组织和正常口腔黏膜组织中的表达差异,以及B7-H3对口腔鳞癌细胞生物学的影响。方法:RT-qPCR、免疫组化检测B7-H3在口腔鳞癌组织和正常口腔黏膜组织的表达差异;构建B7-H3腺病毒表达载体,感染人舌鳞癌细胞Tca8113,CCK-8、PI染色流式细胞仪检测B7-H3高表达对Tca8113细胞增殖和细胞周期的影响。采用SPSS11.0软件包对数据进行统计学处理。结果:RT-qPCR结果显示,口腔鳞癌组织B7-H3 mRNA表达为3.021±0.2310,显著高于正常口腔黏膜组织(0.6002±0.1010);与对照组比较,10、50、100感染复数组在作用24h呈现促进细胞增殖作用,S期细胞比例显著增加,72h时增殖作用最强(P<0.05);与感染复数1组比较,同时间段50、100组促细胞增殖作用和S期细胞比例显著增加(P<0.05),50与100组比较无显著差异(P>0.05)。结论:口腔鳞癌组织B7-H3mRNA和蛋白表达显著高于正常口腔黏膜组织;B7-H3高表达,可促进口腔鳞癌细胞增殖。

PURPOSE： To investigate the differences of B7-H3 expression in oral squamous cell carcinoma tissues and normal oral mucosa, and the function of B7-H3 expression on the biology of oral cancer cells. METHODS： B7-H3 expression in oral squamous cell carcinoma tissues and normal oral mucosa was detected by RT-qPCR and immunohistochemical assay; B7-H3 adenovirus expression vector was constructed, and transfection with Tca8113 cells, then the cell proliferation and cell cycle were evaluated by MTT and flow cytometry（PI staining） assay, respectively. The data was analyzed with SPSS 11.0 software package. RESULTS： RT-qPCR results showed that the B7-H3 mRNA expression （3.021±0.2310） in oral squamous cell carcinoma was significantly higher than normal oral mucosa （0.6002± 0.1010, P〈0.05）; After B7-H3 adenovirus expression vector transfecting Tca8113 cells for 24h, compared with the control group, cell proliferation and S-phase fraction of 10, 50, 100 MOI group increased significantly （P〈0.05）; At the same time, compared with 1 MOI group, cell proliferation and S-phase fraction of 50, 100 MOI groups increased significantly （P 0.05）, however, significant difference was not observed between 50 and 100 MOI group （P〈0.05）. CONCLUSION： In oral squamous cell carcinoma tissues, B7-H3 mRNA and protein expression were significantly higher than normal oral mucosa; in vivo, B7-H3 high expression can promote the proliferation of oral squamous cell carcinoma cells.