鱼藤酮染毒大鼠纹状体某些生化指标的变化

Some bio-chemical indice change of rats striatum poisoned by rotenone

目的观察鱼藤酮染毒对大鼠纹状体某些生化指标的影响。方法采用微量渗透泵背部埋植法观察不同剂量鱼藤酮对大鼠纹状体的影响,利用Fluoro-JadeB复合荧光尼氏染色观察染毒大鼠纹状体神经细胞的改变情况,高效液相色谱法(HPLC)检测纹状体ATP、ADP和AMP含量,采用生化实验分析Na+-K+-ATP酶和Ca2+-ATP酶的活性。结果染毒大鼠纹状体内出现大量的阳性变性神经细胞,而溶剂对照组未出现类似改变。与溶剂对照组比较,2.0和4.0mg/kg鱼藤酮组大鼠纹状体ATP含量出现显著性降低(P<0.01),ADP和AMP含量则相对升高;Na+-K+-ATP酶与Ca2+-ATP酶的活性随染毒剂量的增大出现不同程度的抑制,差异具有统计学意义。结论鱼藤酮可导致纹状体ATP含量降低及Na+-K+-ATP酶与Ca2+-ATP酶活性被抑制。

Objective To observe some of bio-chemical indice of rats striatum poisoned by rotenone.Methods Apply the method of back-implantation mini-effusion pump to observe influence of different concentration rotenone on the rat striatum.Utilize Fluoro-Jade B combined Fluorescent dying to observe the change conditions of the neural cells of the poisoned rat striatum.HPLC is used to measure contents of ATP,ADP,and AMP in striatum.Apply bio-chemical lab to analyze the activities of Na^ ＋ -K^ ＋ -ATPase and Ca^2 ＋ -ATPase.Results There appeared a large quantity of positive degenerative neurons in the poisoned rats striatum,but the solution control group did not show similar change.Compared with the solution control group,the ATP contents in the rat striatum of the 2.0mg /kg and 4.0mg /kg rotenone groups were significantly decreased（P〈0.01）,while the contents of ADP and AMP were relatively raised.With the increase of the poison contents,the activities of Na^ ＋ -K^ ＋ -ATPase and Ca^2 ＋ -ATPase were inhibited to some degrees.The difference has statistical significance.Conclusion Rotenone could cause the decrease of the ATP content and inhibit activities of Na^ ＋ -K^ ＋ -ATPase and Ca^2 ＋ -ATPase.