期刊文献+

生物大分子在纳米微孔中迁移运动的研究进展 被引量:2

Progress in study on translocation of biomacromolecules through a nanopore
下载PDF
导出
摘要 DNA等带电生物大分子通过各种生物膜上的纳米微孔的迁移是生命现象中的关键运动步骤之一,对这种迁移过程的研究不仅有助于了解生命运动的分子机理,在快速DNA测序、基因治疗和药物传输的控制等方面也有潜在应用价值。本文介绍了近年来关于DNA分子通过纳米微孔迁移过程在实验测定、理论分析和计算机模拟等方面的研究进展,重点关注迁移动力学及其机理,特别是迁移时间和分子链长间的标度关系等。 Translocation of charged biological macromolecules such as DNA across biological membranes through a nanopore is one of the most crucial elementary processes of life.Study on the translocation of DNA is helpful to understand the molecular mechanism of life and explore its potential application in rapid DNA sequencing,gene therapy,drug delivery and so on.In this paper,the progresses in the investigation of translocation process of DNA through nanopore by using single-molecule electrophysiology experiments,theory of polymer transport and molecular simulations are reviewed.The translocation dynamics of DNA and its mechanism are paid more attention to,especially,the scaling relationship of translocation time with molecular weight.
出处 《化工学报》 EI CAS CSCD 北大核心 2010年第10期2501-2509,共9页 CIESC Journal
基金 国家自然科学基金项目(20736002,20706013) 化学工程联合国家重点实验室开放课题项目(SKL-ChE-09C02)~~
关键词 DNA 蛋白质 迁移 纳米微孔 分子模拟 DNA protein translocation nanopore molecular simulation
  • 相关文献

参考文献61

  • 1Muthukumar M. Mechanism of DNA transport through pores. Annu. Rev. Bioph ys. Biomol. Struct. , 2007, 36 435 -450.
  • 2Wickner W T, Lodish H F. Multiple mechanisms of protein insertion into and across membranes. Science, 1985, 230: 400- 407.
  • 3Kasianowicz J J, Brandin E, Branton D, Deaner D W. Characterization of individual polynucleotide molecules using a membrane channel. Proc. Natl. Acad. Sci. USA, 1996, 93: 13770-13773.
  • 4Meller A, Nivon L, Branton D. Voltage-driven DNA translocations through a nanopore. Phys. Rev. Lett. , 2001, 86 (15) : 3435-3438.
  • 5Meller A, Nivon L, Brandin E, Golovchenko J, Branton D. Rapid nanopore discrimination between single polynucleotide molecules. Proc. Natl. Acad. Sci. USA,2000, 97 (3): 1079- 1084.
  • 6Sutherland T C, Long Y T, Stefureac R I, Bediako Amoa I, Kraatz H B, I.ee J S. Structure of peptides investigated by nanopore analysis. Nana Letters, 2004, 4 (7) : 1273- 1277.
  • 7Stefureac R, Long Y T, Kraatz H B, Howard P, Lee J S. Transport of R helical peptides through R-hemolysin and aerolysin pores. Biochemistry, 2006, 45 (30): 9172 -9179.
  • 8Zhao Q T, Jayawardhana D A, Wang D Q, Guan X Y. Study of peptide transport through engineered protein channels. J. Phys. Chem. B, 2009, 113 (11):3572-3578.
  • 9Dekker C. Solid-state nanopores. Nat. Nanotech. , 2007, 2: 209 -215.
  • 10Li J L, Stein D, McMullan C, Branton D, Aziz M J, Golovchenko J A. Ion-beam sculpting at nanometre length scales. Nature, 2001, 412:166 -169.

同被引文献5

引证文献2

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部