尖吻蝮蛇血凝酶药效评价及其作用机制

Evaluation of hemostatic effects of Haemocoagulase Agkistrodon and the mechanisms

目的:研究注射用尖吻蝮蛇血凝酶(Haemocoagulase Agkistrodon,HCA,商品名苏灵)的止血效果及其作用机制。方法:通过体内外形成血栓实验,了解药物的凝血效果及其是否激活凝血因子ⅩⅢ;通过HCA对纤维蛋白原的裂解作用探讨其凝血机制。结果:HCA作用下体内外形成血栓的大小与立止血相差不大,差异无统计学意义;蛋白凝胶电泳图显示HCA作用12 h后纤维蛋白原α亚基带消失;高效液相色谱图显示在8.8 min处有明显的峰出现。结论:HCA具有良好的止血效果,且不激活凝血因子ⅩⅢ;HCA能水解纤维蛋白原的α亚基,裂解出A肽片段(FpA),形成纤维蛋白单体(αBβγ),并聚合成纤维蛋白多聚体,发挥止血作用。

Objective： To evaluate the hemostatic effect of Haemocoagulase Agkistrodon（HCA,trade name： Suling） for injection and the mechanisms.Methods： In the in vivo and in vitro experiments of thrombosis,the hemostatic effects of HCA and activation of coagulation factor ⅩⅢ in blood coagulation were evaluated.The mechanisms were investigated by determining the ability of HCA to degrade fibrinogen.Results： HCA had the effect similar to the positive control reptilase,but the difference was not statistically significant.SDS-polyacrylamide gel electrophoresis showed that the α-chain disappeared after 12 h incubation with HCA,and high performance liquid chromatography showed an obvious peak at 8.8 min.Conclusion： HCA has a good hemostatic effect without activating coagulation factor ⅩⅢ.It exerts hemostatic effect by hydrolyzing α-chain of fibrinogen,releasing A peptide fragment（FpA） to generate the fibrin monomers（αBβγ）2 and polymerizing them for hemostasis.