利用毛细管电泳法测定乳品中β-乳球蛋白含量的研究

Detection of β-Lacto Globulin in Milk Products by Capillary Electrophoresis

目的用毛细管电泳技术检测乳品中β-乳球蛋白含量。方法将样品经缓冲液处理,选择直径为70μm,有效长度600mm的未涂层石英毛细管柱;柱温为40℃;缓冲溶液为柠檬酸缓冲液(pH为3.0±0.2);工作电压为27kV;检测波长为214nm;进样时间为5s,进行检测。结果β-乳球蛋白回收率为75.2%～105.2%,相对标准偏差(RSD)为1.09%～3.10%;最低检出限为0.001mg/ml。结论该方法简便、准确、灵敏度高,是快速检测乳品中β-乳球蛋白含量较适合的分析方法。

Objective To establish a capillary electrophoresis method for detecting the content of β-lacto globulin in dairy products.Method Samples were handled with buffers;an uncoated neutral capillary column in the diameter of 70 μm and the effective length of 600 mm was used.The samples were detected under the condition of capillary temperature at 40 ℃;citric acid buffer solution at pH 3.0 ± 0.2,operating voltage at 27 kV,detection wavelength at 214 nm,and injection time for 5 s.Results The average recovery was 75.2%-105.2% and the relative standard deviation （RSD） was 1.09%-3.10% for β-lacto globulin.The detection limit was 0.001 mg /ml.Conclusion The method is simple,accurate and sensitive.It is suitable for fast screening and quantitative monitoring β-lacto globulin in dairy products.