粗针督脉留针治疗对迟发型变态反应性小鼠的影响

Effect of thick needle piercing and retaining on Governor Vessel on mice with delayed type hypersensitivity

目的:观察粗针督脉留针治疗对迟发型变态反应性小鼠模型的影响。方法:利用二硝基氟苯(DNFB)诱发小鼠右耳变态反应性接触性皮炎,以粗针为治疗组,西替利嗪组、氢化可的松组、模型组为对照组,计算治疗后左右耳重量差、诱发皮炎前后右耳厚度差、治疗后左右耳厚度差,做小鼠右耳病理切片观察局部淋巴样单核细胞浸润情况,用酶联免疫吸附试验(ELISA)法测量小鼠血清中的白细胞介素-2(IL-2)、白细胞介素-10(IL-10)、白细胞介素-12(IL-12)、T淋巴细胞干扰素(IFN-γ)4种细胞因子。结果:粗针组在影响血清中相关细胞因子的浓度及改善小鼠耳组织病理情况等方面均优于模型组及西替利嗪组。结论:粗针督脉留针治疗迟发型变态反应性小鼠有确切的疗效。

Objective：To observe the effect of thick needle piercing and retaining on Governor Vessel（i.e.Du meridian） on mice model with delayed type hypersensitivity（DTH）.Methods：Dinitrofluorobenzene（DNFB）was applied to mice＇s right ear to induce allergic contact dermatitis.The mice receiving subsequent thick needle piercing were classified into treatment group,and control groups were divided into cetirizine group,hydrocortisone group,and model group.The weight differences between both ears after treatment,the thickness difference of the right ear before and after induction of dermatitis,as well as the thickness difference between both ears after treatment were measured thereafter.Athological section was done afterward on mice＇s right ear to observe the region lymphoid monocyte infiltrating condition,and mice blood serum was processed using the enzyme-linked immunoassay（ELISA）technique to observe the serum cytokine level including IL-2,IL-10,IL-12 and IFN-gamma.Results：The effects of thick needle piercing in terms of affecting the concentration of related serum cytokine and improving mice＇s ear tissue histopathological appearance were found to be better than those observed in both model and cetirizine groups.Conclusion：Thick needle piercing and retaining on Governor Vessel on mice with delayed type hypersensitivity show an exact therapeutic effect.