2型糖尿病伴牙周炎病人血清HbA1c、龈沟液IL-1β和TNF-α的检测

Serum HbA1c,gingival crevicular fluid IL-1 β and TNF-α Detection in type 2 diabetic patients with periodontitis

目的:研究2型糖尿病伴牙周炎病人、单纯牙周炎病人、牙周健康者龈沟液(gingival cervi-cularfluid,GCF)白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)水平及其关系。方法:选取单纯牙周炎病人35例,2型糖尿病伴牙周炎病人35例,另选取单纯健康者30例,检查并记录探诊出血阳性率、探诊深度和附着水平丧失。运用高效液相层析法、ELISA法、放射免疫法分别测定各组血清糖化血红蛋白(HbA1c)、龈沟液IL-1β和TNF-α的水平。结果:2型糖尿病合并牙周炎组血清糖化血红蛋白、龈沟液IL-1β和TNF-α的水平均明显高于单纯牙周炎组、正常对照组(P<0.05);单纯牙周炎组与正常对照组相比,除血清糖化血红蛋白水平无显著性差异外(P>0.05),龈沟液中IL-1β和TNF-α水平均有显著性差异(P<0.05)。结论:IL-1β、TNF-α可能在牙周炎对2型糖尿病的影响中起一定的作用。

AIM ： Serum glycosylated hemoglobin （ HbA1 c）, gingival crevicular fluid （GCF） interleukin- 1 β （IL-1β） and tumor necrosis factor-α （TNF-α） levels were measured in periodontitis patients with type 2 diabetes, simple periodontitis patients and periodontally healthy individuals so as to evaluate the relationship of these parameters with periodontitis. METHODS： Thirty five periodontitis patients, 35 type 2 diabetic patients with periodontitis and 30 periodontally healthy individuals were included in this study. Serum HbAlc was measured by high performanee liquid chromatography. GCF IL-1β and TNF-α levels were determined by ELISA and radioimmunoassay, respectively. RESULTS： Serum HbA1 c, GCF IL-1β and TNF-α levels in Type 2 diabetes mellitus periodontitis patients were significantly higher than those in periodontitis and periodontally healthy group （ P 〈 0.05 ）. GCF IL-1β and TNF-α levels in periodontitis patients were significantly higher than those in healthy individulas （P 〈 0.05 ）. However, no significant difference was found in serum HbAlc between the 2 groups （P 〉 0.05）. CONCLUSION： IL-1 β and TNF-α might play important roles in the interaction between periodontitis and diabetes.