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蛋氨酸脑啡肽对树突状细胞表型及功能的影响 被引量：7

Regulation on phenotypic and functional maturation of dendritic cell by met-enkephalin
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摘要 目的:通过体外细胞株研究蛋氨酸脑啡肽(Methionine-enkephalin,MENK)对树突状细胞(Dendritic cell,DC)功能调节的机制,为进一步阐明蛋氨酸脑啡肽的免疫活性提供依据。方法:应用扫描电镜技术、酸性磷酸酶活性检测、流式细胞仪检测技术、酶联免疫吸附试验检测DC表型和功能的各种指标。结果:本实验应用小鼠髓系DC2.4细胞株,电镜观察DC的超微结构,可见10-12mol/L蛋氨酸脑啡肽处理组DC突起增多,形态上更加成熟;DC表面MHCⅡ、CD86和CD40的表达增加;酸性磷酸酶活性降低;DC白细胞介素12(IL-12)的表达增加(P<0.05)。结论:本实验结果证明了适宜浓度的蛋氨酸脑啡肽促进小鼠髓系DC2.4的表型及功能的成熟。 Objective：To explore the mechanism of immunomodulatory effects of methionine-enkephalin（MENK）on dendritic cells（DC）.Methods：We used scanning electronic microscope for DC morpholopy,assay for acid phosphatas activity,flow cytometry（FCM） and ELISA to study the effects of DC by MENK.Results：MENK（10-12mol/L） could increase the expression of MHC classⅡ,CD86 and CD40 molecules on DC surface（P0.05）.MENK-treated DCs secreted higher level of IL-12（P0.05）.MENK-treated DC displayed a more matured morpholopy,with long protrusions,while untreated-DCs displayed shorter protrusions.MENK depresses the activity of acid phosphatase in DCs,indicating more maturation.Conclusion：Suitable concentration of MENK can promote the phenotypic and functional maturation of DC markedly.
作者 王宁 夏燕杰 单风平
机构地区 中国医科大学基础医学院免疫学教研室
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2010年第9期814-817,共4页 Chinese Journal of Immunology
基金 国家自然科学基金资助项目(30771990)
关键词 蛋氨酸脑啡肽 树突状细胞 表型 功能成熟 影响 Methionine-enkephalin Dendritic cells Phenotype Functional maturation Effect
分类号 R392.12 [医药卫生—免疫学]
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参考文献14

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共引文献8

同被引文献101

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引证文献7

二级引证文献27

中国免疫学杂志
2010年 第9期

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