摘要
采用荧光光谱、紫外-可见光谱和表面增强拉曼光谱研究了黄芩素和黄芩苷与人血清白蛋白的相互作用,得到了分子间的结合状态和吸附形态方面的信息.通过比较黄芩素和黄芩苷的普通拉曼和表面增强拉曼光谱,确立了黄芩素和黄芩苷的正常构象,同时考察了结合上人血清白蛋白后不同浓度黄芩素和黄芩苷的吸附方式差别.
The fluorescence,ultraviolet-visible and surface-enhanced Raman scattering spectroscopy were applied to study the interaction between baicalein/baicalin and human serum albumin,as the subsequent consequence,the information of intermolecular adsorption and orientation were obtained. By the comparison of norml Ramn and surface-enhanced Raman scattering spectroscopy,the normal conformation of baicalein and baicalin were confirmed. Besides that,we also studied the orientated difference between baicalein and baicalin after their combining to human serum albumin. In addition,the state of freedom ions on the binding of baialein /baicalin and human serum were also investigated in physiological conditions,which is the most accessible work of simulation in vitro.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2010年第9期1834-1840,共7页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20921003
20973034)
国家科技支撑计划(批准号:2007BAI38B03)
全国高校学科创新引智工程(批准号:B06009)资助
关键词
黄芩素
黄芩苷
人血清白蛋白
荧光光谱
紫外-可见光谱
表面增强拉曼光谱
Baicalein
Baicalin
Human serum albumin
Fluorescence spectrum
Ultraviolet-visible spectrum
Surface enhanced Raman scattering spectroscopy