黄芩素和黄芩苷与人血清白蛋白相互作用的光谱研究

Studies on the Interaction for Baicalein and Baicalin with Human Serum Albumin by Spectroscopic Methods

采用荧光光谱、紫外-可见光谱和表面增强拉曼光谱研究了黄芩素和黄芩苷与人血清白蛋白的相互作用,得到了分子间的结合状态和吸附形态方面的信息.通过比较黄芩素和黄芩苷的普通拉曼和表面增强拉曼光谱,确立了黄芩素和黄芩苷的正常构象,同时考察了结合上人血清白蛋白后不同浓度黄芩素和黄芩苷的吸附方式差别.

The fluorescence,ultraviolet-visible and surface-enhanced Raman scattering spectroscopy were applied to study the interaction between baicalein/baicalin and human serum albumin,as the subsequent consequence,the information of intermolecular adsorption and orientation were obtained. By the comparison of norml Ramn and surface-enhanced Raman scattering spectroscopy,the normal conformation of baicalein and baicalin were confirmed. Besides that,we also studied the orientated difference between baicalein and baicalin after their combining to human serum albumin. In addition,the state of freedom ions on the binding of baialein /baicalin and human serum were also investigated in physiological conditions,which is the most accessible work of simulation in vitro.