双阳离子咔唑衍生物与DNA相互作用的光谱研究

Interaction Between Dicationic Carbazole Derivatives and Nucleic Acid by Spectrum

合成了一种新型的双阳离子咔唑衍生物3,6-双(1-羟乙基-4-烯基吡啶)咔唑碘盐(3,6-BHVC),并对其进行了表征.利用紫外-可见吸收光谱、圆二色谱、单光子和双光子荧光发射光谱探讨了该化合物与DNA的结合方式.所有光谱测试结果表明,该化合物在[磷酸根]/[染料]比值较低的条件下主要以插入方式与DNA结合,在该比值较高的条件下主要以沟槽结合方式为主.表明咔唑衍生物可以成为一种探索具有高结合能力双光子DNA探针的基本结构.

In this paper,3,6-bis （ 1-hydroxyethyl-4-vinylpyridinium） carbazole diiodide （ 3,6-BHVC） ,a novel dicationic carbazole derivative,was synthesized and characte-rized. Using electronic absorption spectra, circle dichroism spectra and one-and two-photon emission spectra,the binding modes between 3,6-BHVC and DNA were studied. All spectral results show that 3,6-BHVC intercalates into DNA base pairs at lower ratio of [phosphate]/[3,6-BHVC]and binds in DNA groove at higher ratio. This indicates that carbazole derivative can act as a primary structure to explore two-photon DNA probes with high binding affinity.