期刊文献+

六氯环三磷腈交联寡聚乙烯亚胺的制备及基因载体应用 被引量:9

Synthesis and Characterization of the Hexachlorotriphosphaxene Cross-linked Oligo( ethyleneimine) as Gene Carrier
下载PDF
导出
摘要 将六氯环三磷腈与分子量为600的超支化寡聚乙烯亚胺在干燥氯仿中反应,合成了可降解的交联型聚合物.利用1HNMR表征了聚合物的结构,并用GPC测试了聚合物的分子量.研究了其作为非病毒基因载体的性能,聚合物载体与DNA形成的复合物颗粒粒径为150nm,Zeta电位为30~40mV,凝胶阻滞电泳显示聚合物/DNA在质量比为0.4时能够将DNA完全阻滞.体外转染实验结果表明,载体对HeLa细胞的最佳转染效率为PEI-25K的3倍;聚合物浓度为20μg/mL时,细胞存活率仍然大于80%,材料的细胞毒性低,生物相容性好,具有良好的生物医学应用前景. Hyperbranched oligo( ethyleneimine) with molecular weight of 600 was carried out reaction with hexachlorotriphosphazene in anhydrous chloroform to synthesize a degradable and cross-linked polymer as a non-viral gene carrier. The 1H NMR spectra confirmed the formation of the polymer and its average molecular weight was obtained by GPC with HAc/NaAc buffering solution of pH = 4. 4 as fluent phase. The polymer compressed DNA into stable complexes in diameter of about 150 nm and zeta potential of 30—40 mV measured by dynamic light scattering. Besides,when the mass ratio of polymer and DNA was 0. 4,the plasmids were fully retarded in 1% agarose gel by gel retardation electrophoresis. The cytotoxicity and transfection efficiency were characterized by HeLa cells in vitro. It was indicated that the best transfection efficiency of polymer with lower cytotoxicity was three times higher than that of PEI-25K. Therefore,the synthesized polymer may have good potential applications as non-viral gene carriers.
出处 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2010年第9期1896-1900,共5页 Chemical Journal of Chinese Universities
基金 国家自然科学基金(批准号:50873102 50733003 A3项目20621140369) 科技部项目(批准号:2007DFR50200) 吉林省科技发展计划(批准号:20090128 20096018)资助
关键词 六氯环三磷腈 寡聚乙烯亚胺 基因载体 转染 细胞毒性 Hexachlorotriphosphazene Oligo( ethyleneimine) Gene carrier Transfection Cytotoxicity
  • 相关文献

参考文献22

  • 1Green J. J., Langer R., Anderson D. G.. Accounts Chem. Res. [J], 2008, 41(6) : 749-759.
  • 2Wong S. Y. , Pelet J. M. , Putnam D.. Prog. Polym. Sci. [J] , 2007, 32(8/9) : 799-837.
  • 3Morille M., Passirani C., Vonarbourg A., ClaVreul A., Benoit J. P.. Biomaterials[ J], 2008, 29(24/25) : 3477-3496.
  • 4Boussif O. , Lezoualch F., Zanta M. A., Mergny M. D., Scherman D., Demeneix B., Behr J. P.. P. N. A. S. [J], 1995, 92(16) : 7297-7301.
  • 5Lungwitz U. , Breunig M. , Blunk T. , Goepferich A.. Eur. J. Pharm. Biopharm. [J] , 2005, 60(2) : 247-266.
  • 6Tian H. Y. , Xiong W. , Wei J. Z. , Wang Y. ,Chen X. S. , Jing X. B. , Zhu Q. Y.. Biomaterials[ J] , 2007, 28(18) : 2899-2907.
  • 7田华雨,夏加亮,林浩,陈磊,陈学思,李悦生,景遐斌.两亲性线性-超支化多臂共聚物在水溶液中自组装为阳离子囊泡的研究[J].高等学校化学学报,2006,27(9):1771-1774. 被引量:5
  • 8FUHui-Li(付慧莉) CHENGSi-Xue(程巳雪) ZHUORen-Xi(卓仁禧).高分子学报,2009,(2):97-103.
  • 9Forrest M. L. , Koerber J. T. , Pack D. W.. Bioconjugate Chem. [J] , 2003, 14(5) : 934-940.
  • 10AhnC. H., ChaeS. Y., BaeY. H., KimS. W.. J. Control. Release[J], 2002, 80(1 3): 273-282.

二级参考文献14

  • 1李丽颖,孙平川,要旸,陈铁红,李宝会,金庆华,丁大同.聚(L-丙氨酸)-聚羟乙基谷氨酰胺双嵌段两亲性共聚多肽的合成及其在水溶液中的自组装[J].高等学校化学学报,2005,26(8):1548-1551. 被引量:7
  • 2Xiong X. Y., Tam K. C., Gan L. H.. Macromolecules[J], 2003, 36(26): 9979-9985
  • 3Yu Y. , Zhang L. , Eisenberg A.. Macromolecules[J] , 1998, 31(4) : 1144-1154
  • 4Johnson J. M. , Ha T. , Chu S. et al. Biophysical Journal[J] , 2002, 83(6) : 3371-3379
  • 5Kataoka K. , Harada A. , Nagasaki Y.. Advanced Drug Delivery Reviews[ J ] , 2001 , 47 (1) : 113--131
  • 6Yokoyama M. , Fukushima S. , Uehara R. et al.. Journal of Controlled Release[J] , 1998, 50(1-3) : 79-92
  • 7Brannan A. K., Bates F. S.. Macromolecules[J] , 2004, 37(24): 8816-8819
  • 8Vriezema D. M., Hoogboom J., Velonia K. et al.. Angewandte Chemie-Intemational Edition[J], 2003, 42(7) : 772-776
  • 9Yan D. Y , Zhou Y. F. , Hou J.. Science[J] , 2004, 303(5654) : 65-67
  • 10Zhou Y. F., Yan D. Y.. Angewandte Chemie-International Edition[J], 2004, 43(37): 4896-4899

共引文献4

同被引文献126

引证文献9

二级引证文献18

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部