对氧磷脂酶1、载脂蛋白E基因多态性与2型糖尿病患者氧化型低密度脂蛋白水平相关

Association of paraoxonase 1 and ApoE gene polymorphisms with plasma Ox-LDL level in type 2 diabetic patients

采用限制性片段长度多态性（RFLP）检测福建地区192例2型糖尿病组、116例单纯动脉粥样硬化（AS）患者和105名正常人对氧磷脂酶1（PON1）、载脂蛋白E（ApoE）基因多态性,血清氧化型低密度脂蛋白（Ox-LDL）水平采用酶联免疫吸附法检测.结果 显示,2型糖尿组Ox-LDL高于单纯AS组及正常对照组[（754.2±279.9对526.1±186.2和421.1±163.2）μg/L,P〈0.01].在2型糖尿患者中,PON1基因QQ型Ox-LDL水平高于QR和RR型[（846.6±147.5对763.4±126.7和713.2±132.4）μg/L,P〈0.01];ApoE基因ε3/4＋ε4/4型Ox-LDL水平高于ε3/3和ε2/2＋ε2/3型[（824.3±173.5对741.6±182.5和718.3±167.5）μg/L,P〈0.05],而在单纯AS患者中未发现这种差别.多元同归分析显示,病程、PON1、ApoE是2型糖尿患者血浆Ox-LDL增高的危险因素.

The polymorphisms of paraoxonase 1 （ PON 1 ） and ApoE gene in 192 patients with type 2diabetes mellitus （DM group） , 116 cases of atherosclerosis （AS） without DM （AS group）, and 105 normal subjects （NC group ） were detected by means of PCR-restriction fragment length polymorphism. The serum concentration of Ox-LDL was determined by enzyme linked immunosorbent assay. The results showed that plasma Ox-LDL level in DM group was higher than those in AS and NC groups[（754.2±279.9 vs 526. 1±186.2 and 421.1 ± 163.2 ）μg/L, P〈0.01]. Ox-LDL level in the patients with QQ genotype of PON 1 was higher than those in QR and RR genotypes in type 2 DM[（846.6±147.5 vs 763.4±126. 7 and 7 1 3.2 ±132.4 ） μg/L, P〈0. 0l],and Ox-LDL level in the patients with ε3/4＋ ε4/4 genotype of ApoE was also higher than those in ε3/3 and ε2/2 ＋ ε2/3 geneotypes in type 2 DM[（ 824.3 ± 173.5 vs 741.6± 182. 5 and 718.3 ± 167.5 ） μg/L, P〈0.05], but the difference was not found in atherosclerosis without DM. Multiple regression analysis showed that disease duration, PON1 and ApoE genotypes were the independent predictors of plasma Ox-LDL level in patients with type 2 DM.