急性运动对小鼠心肌KCNE1表达的影响

Effect of acute exercise on expression of KCNE1 in mouse cardiac muscle

目的探讨急性游泳运动对小鼠心肌电压依赖性钾离子通道E家族成员1(KCNE1)表达水平的影响。方法雄性BALB/c小鼠42只,随机分7组,每组6只。非运动为对照组,余6组于急性游泳运动30min后即刻(A组)、1h(B组)、3h(C组)、6h(D组)、12h(E组)和24h(F组)取心肌组织;用RT-PCR和Westernblot技术分别检测各组小鼠心肌KCNE1的表达水平。结果与对照组相比,A、C和D组心肌KCNE1表达水平明显升高(P<0.05)。结论急性运动引起心肌KCNE1表达水平一过性升高,可能是交感紧张增强后心脏电生理活动发生改变的原因之一。

Objective To investigate the effect of acute exercise on expression of KCNE1（potassium voltage-gated channel subfamily E member 1）in mouse cardiac muscle.Methods Forty-two male BALB/c mice were randomly assigned to seven groups with 6 rats each.The rats of control group did not receive exercise as the controls.The mice of 6 exercise groups were forced to complete swimming for 30min.The harts were taken at the times of immediately,1,3,6,12,or 24h after exercise for groups of A,B,C,D,E and F,respectively.KCNE1 expression levels of the myocardium were detected by RT-PCR and Western blot.Results The expression of KCNE1 mRNA was higher in groups of A,C and D than that in control group（P0.05）.Conclusion The acute exercise increases the expression of KCNE1 in the myocardium,which may be one of the causes for sympathetic stimulation-induced chages in cardiac electrophysiological activity.