Occludin表达与胃癌发生发展的相关性

Relationship between the expression of occludin and tumor genesis and development in the gastric carcinoma

目的:探讨Occludin mRNA、蛋白表达水平与胃癌发生发展、侵袭转移的关系。方法:应用组织芯片免疫组化染色和实时荧光定量PCR的方法,检测occludin在胃腺癌组织中的表达并分析其与临床特征的相关性。结果:免疫组织化学检测显示,Occludin在癌旁正常胃黏膜上皮中阳性表达率高于胃腺癌组织(96.9%vs85.4%,84.6%,89.2%,78.5%)(P<0.05);Occludin的表达水平与胃腺癌TNM分期和分化程度有关(P<0.05)。实时荧光定量PCR的检测结果显示,Occludin mRNA在胃癌组织和癌旁正常胃组织中的表达无明显差异(P>0.05)。结论:Occludin蛋白的表达水平与胃腺癌的发生发展和侵袭转移呈负相关。

AIM：To investigate the expression of occludin mRNA and protein in the gastric cancer,to seek the relationship between the expression of occludin and tumor genesis and development.METHODS：The expression of occludin was examined in the gastric carcinoma by immunohistochemical technique with the tissue microarray and by real time fluorescence quantitative PCR,and to study the association of occludin expression and clinicopathological features in the gastric adenocarcinomas.RESULTS：The expression levels of occludin protein in the paraneoplastic normal gastric mucosa is obviously higher than that in the carcinoma（96.9% vs 85.4%,84.6%,89.2%,78.5%）（P〈0.05）,the expression level in the gastric adenocarcinomas is related with the histologic differentiation and the grade of TNM （P〈0.05）.The real time fluorescence quantitative PCR show that the expression of occludin mRNA in the gastric cancer is not obviously different from that in paracancerous tissues （P〉0.05）.CONCLUSIONS：The expression levels of occludin protein in the gastric adenocarcinomas is negative correlation with tumor genesis and development.