非小细胞肺癌中c-Met蛋白的表达及预后意义

Expression of c-Met and the prognosis in Non-small cell lung cancer

目的:检测c-Met蛋白、微血管密度在非小细胞肺癌(NSCLC)中的表达,探讨c-Met蛋白表达与预后的关系。方法:运用免疫组织化学SP法对98例NSCLC组织中的c-Met进行检测,用CD34单克隆抗体(mAb)检测微血管密度。结果:c-Met在NSCLC中阳性表达率为63.3%(62/98),Ⅰ～Ⅱ期阳性率为53.6%低于Ⅲ-Ⅳ期阳性率76.2%(P<0.05);c-Met表达与组织学类型、分化程度、年龄、性别均无关(P>0.05)。CD34标记的MVD平均为36.53±10.70条,其中c-Met阴性组28.94±9.62条明显低于c-Met阳性组40.94±8.68条,t检验示P=0.01。Kaplan-Meier生存分析显示:c-Met阴性组1年、3年、5年生存率分别为100%、86.1%、63.9%阳性组分别为75.8%、46.8%、17.7%(P=0.000);Ⅰ-Ⅱ期1年、3年、5年生存率分别为100.0%,78.6%,50.0%;Ⅲ-Ⅳ期1年、3年、5年生存率分别为64.3%,38.1%,14.3%(P=0.01);与性别、年龄、组织类型、分化程度无关(P>0.05)。COX回归分析,c-Met表达及TNM分期均为独立的预后因素(P=0.01)。结论:c-Met的表达与NSCLC的血管形成密切相关,并且是一种预后不良指标。

AIM To investigate the relationship between the expression of c-Met and MVD and the prognosis in non-small cell lung cancer （NSCLC）.METHODS：c-Met protein expression and microvessel density （MVD） was assessed by immunohistochemistry in 98 specimens of NSCLC.Tumor specimens were stained for c-Met and CD 34 （specific MVD marker）.The relationship between the expression of c-Met and MVD,clinicopathological features of NSCLC were analyzed.RESULTS：The positive expression rate of c-Met in NSCLC was 63.3% （62/98）.The c-Met positive percentage of stage Ⅲ-Ⅳ was higher than that of stage Ⅰ-Ⅱ,76.2% vs 53.6%,P=0.022.There was no significant difference between c-Met expression and other clinicopathological features,such as histological type,gender,age,differentiation（P〉0.05）.Average MVD of this study was 36.53±10.70.MVD in NSCLC with c-Met positive expression was higher than that of negative expression,28.94±9.62 vs 40.94±8.68（P=0.000）.The overall survival （OS） of 1-,3-,5-year of patients with negative c-Met （100.0%,86.1%,63.9%） was higher than those with positive c-Met （75.8%,46.8%,17.7%）,P=0.000.OS of 1-,3-,5-year of patients in stageⅠ-Ⅱ（100.0%,78.6%,50.0%） was higher than those in stage Ⅲ-Ⅳ （64.3%,38.1%,14.3%）,P=0.000.There was no remarkable correlation between OS and histological type,gender,age,differentiation（P〉0.05）.Furthermore,multivariate COX regression analysis showed that c-Met expression and TNM stage were independent prognosis（P=0.000）.CONCLUSION：c-Met expression in NSCLC correlated closely with tumor angiogenesis,and poor prognosis.