RECK基因在鼻咽癌中的表达及其临床意义

Expression of RECK gene in nasopharyngeal carcinoma and its clinical significance

目的检测伴有Kazal域的富含半胱氨酸的逆转诱导蛋白(reversion-inducing cysteine-rich protein with Kazal motif,RECK)基因在鼻咽癌(nasopharyngeal carcinoma,NPC)组织中的表达及其与临床病理因素的关系。方法采用原位杂交技术检测60例原发性NPC(颈淋巴结转移组和无颈淋巴结转移组各30例)、10例NPC颈部转移淋巴结(metastastic lymph node,MLN)和20例鼻咽黏膜慢性炎症组织(chronic nasopharyngitis tissue,CNT)中RECK mRNA的表达,并对NPC各临床病理参数进行分析。结果 CNT、NPC和MLN中RECK的阳性表达率分别为85.0%(17/20)、26.7%(16/60)和30.0%(3/10)。与CNT比较,NPC和MLN中RECK表达下调,差异均有统计学意义(P均<0.05)。NPC有淋巴结转移组与无淋巴结转移组RECK阳性表达率分别为13.3%(4/30)和40.0%(12/30),两者比较,差异有显著性(P<0.05)。NPC原发灶中RECK低表达与患者性别、年龄、T分级和临床分期无关(P均>0.05),与颈淋巴结转移、复发和治疗后5年生存期有关(P均<0.05)。结论 RECK基因低表达可能与NPC的进展有关,它可作为评估NPC颈淋巴转移、复发及预后的生物学参考指标。

OBJECTIVE To determine the expression of RECK gene in nasopharyngeal carcinoma （NPC）and its correlation with clinicopathologic features. METHODS In situ hybridization was used to detect the expression of RECK mRNA in 60 NPC samples（30 cases with,30 cases without cervical lymph node metastasis）,10 samples of metastastic lymph node（MLN）and 20 samples of chronic nasopharyngitis tissue（CNT）. RESULTS Positive rates of RECK mRNA expression in CNT,NPC and MLN were 85.0%（17/20）,26.7% （16/60） and 30.0%（3/10）,respectively. The RECK mRNA expression in NPC and MLN were signi？cantly lower than that in CNT（P0.05）. The RECK mRNA expression in NPC with lymph node metastasis was significantly lower than in NPC without lymph node metastasis（13.3% vs. 40.0%,P0.05）. Expression of RECK mRNA in NPC had no correlation with gender,age,T-stage and clinical stage （P0.05）,but it had positive relationship with lymph node metastasis,tumor recurrence and the 5-year survival periods of NPC（P0.05）. CONCLUSION These data suggest that the lower expression of RECK gene may be involved in the development of NPC and may be a good biomarker for evaluating the cervical lymph node metastasis,recurrence and prognosis of NPC.