复方氯胺酮口服液对胃肠动力的影响

Effects of Compound Ketamine Oral Solution on gastrointestinal motility in rats

目的:观察复方氯胺酮口服液(CKOS)对大鼠胃排空、肠转运速度、胃动素水平的影响,进一步评价其临床应用的安全性.方法:选Wister大鼠125只,随机分成5组,每组25只,每组又分成5个时点,每个时点5只.对照组(C组):蒸馏水1mL灌胃;调味剂组:调味剂溶液(含清甜素、巧克力香精、除苦剂)1mL灌胃;咪达唑仑组(MID组):咪达唑仑溶液1mL灌胃,剂量为10mg/kg;氯胺酮组(KET组):氯胺酮溶液1mL灌胃,剂量为100mg/kg;CKOS组:CKOS溶液(含氯胺酮剂量为100mg/kg、咪达唑仑剂量为10mg/kg加调味剂等)1mL灌胃.立刻用0.2%酚红1mL灌胃.分别于0、30、60、120、360min5个时点断头取血2mL,检测血浆胃动素.将30min时点对应的大鼠取出胃肠,观察色素在小肠移行速度,用分光光度计检测胃内色素残留率.结果:C组30、60min胃动素水平分别为219.6ng/L±13.05ng/L、235.4ng/L±22.43ng/L较0点水平178.3ng/L±15.32ng/L有显著的升高(均P<0.01),T组30、60min胃动素较0点也有明显升高(均P<0.01),且与C组对应时点相比,也有显著差异(均P<0.01),表明调味剂能促进胃动素的分泌.KET组、MID组、CKOS组30、60min胃动素水平都低于C组对应时点(均P<0.05或0.01).T组30min胃残留率为30.29%±7.42%比C组30min残留率(46.07%±6.01%)显著减少(P<0.01),T组小肠推进比显著大于C组(P<0.01).KET组、MID组和CKOS组的30min胃残留率显著大于C组(P<0.05或0.01).KET组小肠推进比显著小于C组(P<0.01).结论:CKOS口服减慢大鼠胃液体排空速度;CKOS对胃动素分泌有一定的抑制作用.

AIM：To investigate the effects of Compound Ketamine Oral Solution（CKOS） on gastric emptying rate,intestinal transit rate,and plasma motilin levels in rats and to evaluate the safety of application of CKOS to clinical practice.METHODS：One hundred and twenty-five Wistar rats were randomly divided into five groups（25 rats each）：control group（intragastrically given 1 mL distilled water）,sauce group（intragastrically given 1 mL sauce solution）,midazolam（MID） group（intragastrically given 1 mL MID solution,10 mg/kg）,ketamine（KET） group（intragastrically given 1 mL KET solution,100 mg/kg）,and CKOS group（intragastrically given 1 mL CKOS）.These groups were further divided into five subgroups（five rats each） for testing at different time points.After gastric administration,each rat was intragastrically given 1 mL of 0.2% phenolphthalein.Blood samples were taken at 0,30,60,120 and 360 min to measure plasma motilin levels.The stomach and small intestine of the rats in the 30-min subgroup were removed to measure the rates of gastric emptying and intestinal transit,respectively.RESULTS：In the control group,the motilin levels at 30 and 60 min were significantly higher than that at baseline（219.6 ng/L ± 13.05 ng/L and 235.4 ng/L ± 22.43 ng/L vs 178.3 ng/L ± 15.32 ng/L,both P 0.01）.In the sauce group,the motilin levels at 30 and 60 min were significantly higher than that at baseline and those at corresponding time points in the control group（all P 0.01）.The motilin levels at 30 and 60 min in the KET,MID and CKOS groups were lower than those in the control group at corresponding time points（all P 0.01 or 0.05）.There were no differences in the motilin levels among the KET,MID and CKOS groups.The rates of gastric emptying and intestinal transit were signif icantly higher in the sauce group than in the control group（both P 0.01）.The rates of gastric emptying at 30 min in the KET,MID and CKOS groups were significantly lower than that in the control group（all P 0.01 or 0.05）.The rate of intestinal transit in the KET group was lower than that in the control group（P 0.01）.CONCLUSION：CKOS can slow down gastric emptying in rats.KET and MID can inhibit excretion of motilin.