hMLH1及hPMS2 mRNA在胃癌组织中荧光定量RT-PCR的检测及其临床意义

Clinical significance of detection of hMLH1 and hPMS2 mRNA expression in gastric cancer by real-time fluorescent quantitative RT-PCR

目的:探讨DNA错配修复基因hMLH1及hPMS2在胃癌组织中的表达水平及其临床意义.方法:应用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术对40例胃癌患者的癌组织、40例癌旁胃炎组织及21例慢性胃炎患者的慢性胃炎组织hMLH1 mRNA及hPMS2 mRNA进行定量检测,以三磷酸甘油醛脱氢酶基因(hGAPDH)为内参照.结果:胃癌组织、癌旁组织、慢性胃炎组织中hMLH1 mRNA的相对含量分别是7.23±11.91,3.80±5.13,2.01±1.25,三组相比差异有统计学意义(F=3.272,均P=0.042),胃癌组织中hMLH1 mRNA含量明显高于其他两组,癌旁组织中含量明显高于慢性胃炎组织中的含量(均P<0.05);各组织中hPMS2 mRNA的相对含量分别是0.43±0.35,0.55±0.39,0.32±0.15,3组相比差异有统计学意义(F=3.349,均P=0.039),胃癌组织与癌旁组织中hPMS2mRNA的含量差异无统计学意义,但均高于慢性胃炎组织中的含量(均P<0.05).除hMLH1 mRNA含量在有、无淋巴结转移的胃癌组织中有显著差异(均P<0.05)外,在胃癌组织中hMLH1 mRNA及hPMS2 mRNA相对含量受肿瘤直径、浸润深度、淋巴结转移的影响不大(均P>0.05).结论:胃癌组织和癌旁组织与慢性胃炎组织相比存在hMLH1及hPMS2 mRNA转录差异,这种基因的转录差异可能与胃癌的发生有关,而与胃癌的发展关系不显著.

AIM： To investigate the expression of DNA mismatch repair genes hMLH1/hPMS2 and to analyze their clinical significance in gastric cancer.METHODS： The expression of hMLH1/hPMS2 mRNAs in 40 specimens of gastric cancer and cancer-adjacent mucosa tissue and 21 specimens of chronic gastritis tissue was detected by realtime fluorescent quantitative reverse transcription-polymerase chain reaction （RT-PCR）.The human glyceraldehydes-3-phosphate dehydro-genase （hGAPDH） gene was used for normalization of gene expression levels.RESULTS： The level of hMLH1 mRNA in gastric cancer was significantly higher than those in cancer-adjacent mucosa tissue and chronic gastritis tissue （7.23 ± 11.91 vs 3.80 ± 5.13 and 2.01 ± 1.25,respectively;both P 0.05）.The level of hMLH1 mRNA was significantly higher in cancer-adjacent mucosa tissue than in chronic gastritis tissue （P 0.05）.The level of hPMS2 mRNA was significantly higher in gastric cancer and cancer-adjacent mucosa tissue than in chronic gastritis tissue （0.43 ± 0.35 and 0.55 ± 0.39 vs 0.32 ± 0.15,respectively;both P 0.05）.No significant difference was noted in the level of hPMS2 mRNA between gastric cancer and cancer-adjacent mucosa tissue.The expression levels of hMLH1/hPMS2 mRNAs in gastric cancer showed no significant correlation with tumor size,infiltration degree,and lymph node metastasis.However,the expression level of hMLH1 mRNA was significantly higher in gastric cancer with lymph node metastasis than in that without lymph node metastasis （all P 0.05）.CONCLUSION： The expression levels of hMLH1 /hPMS2 gene in gastric cancer and canceradjacent mucosa tissue are abnormal when compared with that in chronic gastritis.Abnormal transcription of hMLH1/hPMS2 may be related with the genesis of gastric cancer,but not involved in the progression of the disease.