大鼠肝组织冷冻和射频毁损对肾脏形态学和功能影响的实验比较

A comparative study on morphologic and functional changes in kidney after cryoablation or radiofrequency ablation for liver tissue in rats

目的:比较大鼠肝组织冷冻或射频毁损后对肾脏的形态学和功能的影响。方法:SD大鼠随机分成A(n=52)、B(n=42)两组,A组给予冷冻治疗,-196℃液氮浸泡冷冻,治疗时间15～20s;B组给予射频治疗,功率15W,时间60～90s。按预定时间处死。术前和术后1、3、7、14天检测血Cr、BUN变化和尿中红细胞以观察血尿的改变,同时观察肾脏组织学表现。结果:术后1天,两组血Cr(P<0.01)、BUN(P<0.01)均明显升高,尿红细胞也明显增多(P<0.05),但A组变化更显著,术后14天各项指标均恢复正常。术后早期,A组肾小管腔内出现较多的血红蛋白管型,肾小球炎细胞浸润,术后14天恢复正常;B组变化轻微。结论:相同条件下,冷冻治疗引起了严重肾功能损害并发症,而射频治疗影响轻微。在一定条件下,射频是相对安全的。

Objective：To compare the morphologic and functional changes in kidney after cryoablation（CA）or radiofrequency ablation（RFA）for liver tissue in rats.Methods：In this study,ninety-four Sprague-Dawley rats were randomly divided into 2 groups.Group A（n=52）was treated with cryoablation by liquid nitrogen,which was performed in 15-20 s.Group B（n=42）was treated with RFA by using a cooled-tip technique.The energy output was 15 W for（60-90） s.All rats were sacrificed at prescheduled time,serum and tissue samples from the kidney were collected for subsequent analysis.Serum urea nitrogen（BUN）,creatinine（Cr）in blood,and urine erytrocytes were measured prior to and 1,3,7,14 days after surgery.Histologic studies of kidney tissue were compared between two kinds of treatment groups.Results：At 1 day after ablation,serum concentration of BUN（P 〈 0.01）,Cr（P 〈 0.01）,and urine RBC（P 〈 0.05）in both groups in creased evidently,which changed significantly in group A group B.In the early stage,many red-orange（hemoglobin-like）crystals appeared in the renal tubule lumen,and lymphocytes infiltration were found in group A.But these significant changes were not present in renal sections from the rats in group B.Conclusion：Hepatic tissue cryoablation results in severe renal dysfunction,but an approximate volume of liver parenchyma underwent RFA impacts lightly,which could be a safe therapy for liver tumor. 更多